Sequences from confirmation amplicon


Confirmation sequences for line 037C03 and hit At5g12920 are shown.
Line ID 037C03
Locus At5g12920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4079389
Primers used for PCR BD20 - 8474, show primer details
   
Sequence Name 16-K027845-022-037-C03C-8409
GenBank Accession LN493293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K027845-022-037-C03C-8409
GCCATTTACCTTCATTCTTGTGTTCTCTGGCTCTGATCACGTTATGATATTGGTAACGTT
CAGATGAGCCAATTGCCTTTAGCATCTCTACATCTTGCACCGAAGCTGCAGAAAATTGCA
TCTCTTAAGGTAAGTTCTGTCTTTTGTTTTCCATTTCACTTGTTTCATCGTTCAGCAACC
TTTTTCTGCTTCTTTGGTTTTTTTTTTTTTAAAAAAAAATTTGTAATGGGGGAATCATGT
TACCCCCAAAACTTCCAGCAGTTGATCACACTGAAATTTTGGCAGCATGAAAATAAGTTT
CCATAA
   
Sequence Name 17-K027845-022-037-C03C-BD20
GenBank Accession LN493715 [GenBank]
Sequencing primer gene specific primer, BD20: 5'-TCATGGAAACTTATTCTCATGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K027845-022-037-C03C-BD20
TGCTGGAGTTTCGGGGGTACATGATTCCCACATTACAAATTTTTATTTAAAAAAAAAAAA
CCCAAAGAAGCAAAAAAGGGTTGCTGAACGATGAAACAAGTGAAAGGGAAAACAAAAGAC
AGAACTTCCCTTAAGAGATGCATTTTTCTGCACCTTCGGGGCAAGATGTAAAGATGCTAA
AGGCAATTGGCTCATCTGAACGTTCCCAATATCATAACGGGATCAGAGCCAGAAAACACA
AGAATGAAGGTAAAGGGCTTCTTGTCCGGGAAATCTACTGGGATCACCAATGAGTATGAG
GGCCAATATGGAAAAAAAAAAAGAGTAATTCCCATTTTTTTTTCAATTCAAAAATGTAAA
TGCCCCCAGCGTTTTTATAAAAGGAAAGTCCTTTTTGATAAAACGACAAATTACGACCCG
CCGTATTTATGGGCGAAAGCAATAAACAAATTTTTCTAATTCGAAAATCTTTATTTCGAC
GTGTCTACTT
Primers used for PCR FL75 - 8474, show primer details
   
Sequence Name 61-K029745-022-037-C03C-8409
GenBank Accession LN510425 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K029745-022-037-C03C-8409
CATTTACAATTGAATATATCCTGCCATTTGAAATATACTGCCATAGCCAATTCCAGCTCG
CCCCTTGTAAGACTTACATCTTTGCGGCTCTGAAAAGCAGAAGAGTTTCTTCCTCCACGA
AGGTCCCAGATATGAATGATTCCTTCTTTACCAGCTCCAAAAATAGTCTAAATGGTAGAT
GAAAGAAACGCAGTCTAAAATTTTGGGTCCTTACTAAATTTGAAATCAAAGTCTTACTGC
AATTTCAATGATCAGAAGAACTTTACTGGGCTTGGGGATATTTTACTCTATGATTACCTG
ATTATCCACATATATTTGAATACTCTTGATCGAATCATATCTGTCTGTTGA
   
Sequence Name 62-K029745-022-037-C03C-FL75
GenBank Accession LN510661 [GenBank]
Sequencing primer gene specific primer, FL75: 5'-ATGCATTGAACTTTCAACAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K029745-022-037-C03C-FL75
TGTGGATAATCAGGTAATCATAGAGTAAAATATCCCCAAGCCCAGTAAAGTTCTTCTGAT
CATTGAAATTGCAGTAAGACTTTGATTTCAAATTTAGTAAGGACCCAAAATTTTAGACTG
CGTTTCTTTCATCTACCATTTAGACTATTTTTGGAGCTGGTAAAGAAGGAATCATTCATA
TCTGGGACCTTCGTGGAGGAAGAAACTCTTCTGCTTTTCAGAGCCGCAAAGATGTAAGTC
TTACAAGGGGCGAGCTGGAATTGGCTATGGCAGTATATTTCAAATGGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTC

Last Updated on 06.12.2018 16:11