Sequences from confirmation amplicon


Confirmation sequences for line 044H05 and hit At1g48520 are shown.
Line ID 044H05
Locus At1g48520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17940558
Primers used for PCR EV54 - 3144, show primer details
   
Sequence Name 04-K103223-0022-044-H05C-3144
GenBank Accession LN487876 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103223-0022-044-H05C-3144
TCCACATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAGT
AAATAATGAACTTTGGAATTCAACAACTTTGGAATTCAAAACAGGCAAAGCACCAGGCAA
ACCCATACAAACAGGACAAATACTTGTGTTTGGATAAGAACCGTAATTGTTAGAGCAACT
ACAAAAGGCTTTTGTCAATGTAGAGAGCTGAACATGAGTCTCTATCCCAATTACAGCTTC
GTAATCTCTCAGAATCTCATCAAGTTGGTTGCTTTTGTGGTTTTTTGGAGCAACCCTTGT
TGGAGAAGACTGTTTAGCTTCATGGGTCGTCGTTGTTTGGCTTCCACAAGACCTCACGGA
GAAGTTGTTACTCCTTCTCCTTCTTAACAAGCTAGTTCCGAGAAGTGAGAACTGGTTTAG
   
Sequence Name 52-K103230-0022-044-H05C-EV54
GenBank Accession LN507782 [GenBank]
Sequencing primer gene specific primer, EV54: 5'-AGCTAAACCAGTTCTCACTTCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103230-0022-044-H05C-EV54
ACAACTTCTCCGTGAGGTCTTGTGGAAGCCAAACAACGACGACCCATGAAGCTAAACAGT
CTTCTCCAACAAGGGTTGCTCCAAAAAACCACAAAAGCAACCAACTTGATGAGATTCTGA
GAGATTACGAAGCTGTAATTGGGATAGAGACTCATGTTCAGCTCTCTACATTGACAAAAG
CCTTTTGTAGTTGCTCTAACAATTACGGTTCTTATCCAAACACAAGTATTTGTCCTGTTT
GTATGGGTTTGCCTGGTGCTTTGCCTGTTTTGAATTCCAAAGTTGTTGAATTCCAAAGTT
CATTATTTACTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCG
GGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAG
GATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCT
TTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCAT
CTNTGGG

Last Updated on 06.12.2018 16:11