Sequences from confirmation amplicon


Confirmation sequences for line 052D02 and hit At5g09860 are shown.
Line ID 052D02
Locus At5g09860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3070592
Primers used for PCR CW47 - 3144, show primer details
   
Sequence Name 74-K104197-0022-052-D02C-CW47
GenBank Accession LN513067 [GenBank]
Sequencing primer gene specific primer, CW47: 5'-GTGACACGACTTAAAAACCACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K104197-0022-052-D02C-CW47
TGGCAAACATAAAGTTTATGTTGATAGCTATAATAGTAACACTTTTAGCTTTTGGTTCCT
CAAGTATTTGATTTTTTTGTTTGTTTGAGTGTATTTTTAGTGGATTGCAATTGAATCCAA
TGTTTATTAGCTGCGAATATGTGTCTAGTGTTACACTTAGACGATAAATTACGCAAAAGA
ACTTAATTGTATGATGATTTTGTTGAGTATATAGAAATGGAAAGACAGGTAATAAGCTAG
CAGATTTGCACTCATACAAACATATTTAGAAAATACCTTTTAACATAATTTGTTAAACAT
CAATACTTTTGAACTTCATATCCAAAGGATTCCCCATTGAAGAAAGGGTCGACAAACTGA
AAGCGTATTCATGAGACGGGCATAGGAGGATGATCAAGATCAGTGTCCATCAGACCAGCT
TCTTCTGTCTCTCCGTCTTCGATTTGACCGGCCTCTTGACCGTTTTCTGTATCAGAACCA
CCGAGTCTTTGCTGTTCCTCAGGCGTAGTTTAAACCGAAGGCGGGAAACGACAATCTGAT
CCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTG
CGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 84-K104198-0022-052-D02C-3144
GenBank Accession LN514425 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K104198-0022-052-D02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTACGCCTGAGGAAC
AGCAAAGACTCGGTGGTTCTGATACAGAAAACGGTCAAGAGGCCGGTCAAATCGAAGACG
GAGAGACAGAAGAAGCTGGTCTGATGGACACTGATCTTGATCATCCTCCTATGCCCGTCT
CATGAATACGCTTTCAGTTTGTCGACCCTTTCTTCAATGGGGAATCCTTTGGATATGAAG
TTCAAAAGTATTGATGTTTAACAAATTATGTTAAAAGGTATTTTCTAAATATGTTTGTAT
GAGTGCAAATCTGCTAGCTTATTACCTGTCTTTCCATTTCTATATACTCAACAAAATCAT
CATACAATTAAGTTCTTTTGCGTAATTTATCGTCTAAGTGTAACACTAGACACATATTCG
CAGCTAATAAACATTGGATTCAATTGCAATCCACTAAAAATACACTCAAACAAACAAAAA
AATCAAATACTTGAGGAACCAAAAGCTAAAAGTGTTACTATTATAGCTATCAACATAAAC
TTTATGTTTTGCCATCAATATTTATTTAGAATATAAATGAGTGGTTTTT
Primers used for PCR CW46 - 3144, show primer details
   
Sequence Name 65-K104377-0022-052-D02C-CW46
GenBank Accession LN511393 [GenBank]
Sequencing primer gene specific primer, CW46: 5'-TTGATAGGTTTACTGCTGGAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K104377-0022-052-D02C-CW46
ATTTTCTAGGGTAAATTTTCTTCGATTTCGATTTCCCAATGCTTCATATGATTGCTTTTA
AAACCTTTTTCTTTCTTTTTCACAGTTTACTGAAATGGGAATCGAAGGAGTTGTCCCAGT
TGAACTATTGCCGCCTGAGGTCCGGTCCAAATACCAAGCTAAACCAAACGAAAAAGCCAA
ACGAGCGAAGAAAGAAGAAACCAAAGGTGGATCTCATGAAACAGAGGGAAACCAGATTGG
TGTTTCTAATAGTGAAGCTGAAGCTGAAGGAGGAAGGGGTGATGCAGAGACGATGGAGAG
TGATGCTATTGCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 81-K104378-0022-052-D02C-3144
GenBank Accession LN514065 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K104378-0022-052-D02C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCAA
TAGCATCACTCTCCATCGTCTCTGCATCACCCCTTCCTCCTTCAGCTTCAGCTTCACTAT
TAGAAACACCAATCTGGTTTCCCTCTGTTTCATGAGATCCACCTTTGGTTTCTTCTTTCT
TCGCTCGTTTGGCTTTTTCGTTTGGTTTAGCTTGGTATTTGGACCGGACCTCAGGCGGCA
ATAGTTCAACTGGGACAACTCCTTCGATTCCCATTTCAGTAAACTGTGAAAAAGAAAGAA
AAAGGTTTTAAAAAGCAATCATATGAAGCATTGGGAAATCGAAATCGAAGAAAATTTTAC
CCTAGAAAATCCTTCAAGGTCTTGCCGAGCTGTAAACCGAAGACCTTTCCAGCAGTAAAC
CTATCA
Primers used for PCR AM28 - 3144, show primer details
   
Sequence Name 03-K105704-0022-052-D02C-AM28
GenBank Accession LN487487 [GenBank]
Sequencing primer gene specific primer, AM28: 5'-TTGATAGGTTTACTGCTGGAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105704-0022-052-D02C-AM28
TTGAGGATTTTCAGGGTAAATTTTCTTCGATTTCGATTTCCCAATGCTTCATATGATTGC
TTTTAAAACCTTTTTCTTTCTTTTTCACAGTTTACTGAAATGGGAATCGAAGGAGTTGTC
CCAGTTGAACTATTGCCGCCTGAGGTCCGGTCCAAATACCAAGCTAAACCAAACGAAAAA
GCCAAACGAGCGAAGAAAGAAGAAACCAAAGGTGGATCTCATGAAACAGAGGGAAACCAG
ATTGGTGTTTCTAATAGTGAAGCTGAAGCTGAAGGAGGAAGGGGTGATGCAGAGACGATG
GAGAGTGATGCTATTGCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGA
TCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTT
GCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAATCAAATCCA
   
Sequence Name 19-K105705-0022-052-D02C-3144
GenBank Accession LN494760 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K105705-0022-052-D02C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGACATGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCAATAGCATC
ACTCTCCATCGTCTCTGCATCACCCCTTCCTCCTTCAGCTTCAGCTTCACTATTAGAAAC
ACCAATCTGGTTTCCCTCTGTTTCATGAGATCCACCTTTGGTTTCTTCTTTCTTCGCTCG
TTTGGCTTTTTCGTTTGGTTTAGCTTGGTATTTGGACCGGACCTCAGGCGGCAATAGTTC
AACTGGGACAACTCCTTCGATTCCCATTTCAGTAAACTGTGAAAAAGAAAGAAAAAGGTT
TTAAAAAGCAATCATATGAAGCATTGGGAAATCGAAATCGAAGAAAATTTTACCCTAGAA
AATCCTTCAAGGTCTTGCCGAGCTGTAAACCGAAGACCTTTCCAGCATAACACTTATCA

Last Updated on 06.12.2018 16:11