Sequences from confirmation amplicon


Confirmation sequences for line 055E10 and hit At1g01580 are shown.
Line ID 055E10
Locus At1g01580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:211177
Primers used for PCR MJ08 - 8474, show primer details
   
Sequence Name 59-K018493-022-055-E10C-MJ08
GenBank Accession LN509857 [GenBank]
Sequencing primer gene specific primer, MJ08: 5'-TAGTTGACCATTTGCCTTCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K018493-022-055-E10C-MJ08
CAATACTTAGCTTCTCTGGTTCGAGTTTGCTACTGGAAGTTATCGTAAACGGATGCCATT
GCAGCTTTGAAATGCTCGGTATGTTCACAAACATTATGCTCGTCGGGCTATAAACCAACT
CTATAAAACAAAAAGAATAGTTTAGAATTAGATCAATATCCAGTAAGGGTGAACTTTTCA
TATACAATTAGCCAATTGGACAAAAGGTACTCACTAGAGTTTTTGGAGAAAGTGAGCTCC
ATAGTGTCGGAAGGAAGAATCCGAGCAGCGAGCAAGCGAACATTTTCACGTGACTGAAGA
AACCGAAGGAAACGATCGACTAAAAAGATGTAGAAACCAGGGAGAGCGATGAAGGAGAAA
CTTATGCCGACGTGGAGGACAAAGAAGAGCATGAAGACGATGTAGAGATAGTGAGTGTAG
AAGAAGACTTCGATAAAAGTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATTGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 60-K018493-022-055-E10C-8409
GenBank Accession LN510129 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K018493-022-055-E10C-8409
TACTTTTATCGAAGTCTTCTTCTACACTCACTATCTCTACATCGTCTTCATGCTCTTCTT
TGTCCTCCACGTCGGCATAAGTTTCTCCTTCATCGCTCTCCCTGGTTTCTACATCTTCTT
AGTCGATCGTTTCCTTCGGTTTCTTCAGTCACGTGAAAATGTTCGCTTGCTCGCTGCTCG
GATTCTTCCTTCCGACACTATGGAGCTCACTTTCTCCAAAAACTCTAGTGAGTACCTTTT
GTCCAATTGGCTAATTGTATATGAAAAGTTCACCCTTACTGGATATTGATCTAATTCTAA
ACTATTCTTTTTGTTTTATAGAGTTGGTTTATAGCCCGACGAGCATAATGTTTGTGAACA
TACCGAGCATTTCAAAGCTGCAATGGCATCCGTTTACGATAACTTCCAGTAGCAAACTCG
AACCAGAGAAGCTAAGTATTGTTATCAAGAAAGAAGGCAA
Primers used for PCR FM31 - 8474, show primer details
   
Sequence Name 15-K029299-022-055-E10C-8409
GenBank Accession LN492839 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K029299-022-055-E10C-8409
CACTCACTATCTCTACATCGTCTTCTGCTCTTCTTTGTCCTCCACGTCGGCATAAGTTTC
TCCTTCATCGCTCTCCCTGGTTTCTACATCTTCTTAGTCGATCGTTTCCTTCGGTTTCTT
CAGTCACGTGAAAATGTTCGCTTGCTCGCTGCTCGGATTCTTCCTTCCGACACTATGGAG
CTCACTTTCTCCAAAAACTCTAGTGAGTACCTTTTGTCCAATTGGCTAATTGTATATGAA
AAGTTCACCCTTACTGGATATTGATCTAATTCTAAACTATTCTTTTTGTTTTATAGAGTT
GGTTTATAGCCCGACGAGCATAATGTTTGTGAACATACCGAGCATTTCAAAGCTGCAATG
GCATCCGTTTACGATAACTTCCAGTAGCAAACTCGAACCAGAGAAGCTAAGTATTGTTAT
CAAGAAAGAAG
   
Sequence Name 16-K029299-022-055-E10C-FM31
GenBank Accession LN493308 [GenBank]
Sequencing primer gene specific primer, FM31: 5'-TAGTTGACCATTTGCCTTCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K029299-022-055-E10C-FM31
TGCTACTGGAAGTTATCGTAAACGGATGCCATTGCAGCTTTGAAATGCTCGGTATGTTCA
CAAACATTATGCTCGTCGGGCTATAAACCAACTCTATAAAACAAAAAGAATAGTTTAGAA
TTAGATCAATATCCAGTAAGGGTGAACTTTTCATATACAATTAGCCAATTGGACAAAAGG
TACTCACTAGAGTTTTTGGAGAAAGTGAGCTCCATAGTGTCGGAAGGAAGAATCCGAGCA
GCGAGCAAGCGAACATTTTCACGTGACTGAAGAAACCGAAGGAAACGATCGACTAAGAAG
ATGTAGAAACCAGGGAGAGCGATGAAGGAGAAACTTATGCCGACGTGGAGGACAAAGAAG
AGCATGAAGACGATGTAGAGATAGTGAGTGTAGAAGAAGACTTCGATAAAAGTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11