Sequences from confirmation amplicon


Confirmation sequences for line 061C04 and hit At5g66210 are shown.
Line ID 061C04
Locus At5g66210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26457108
Primers used for PCR DL43 - 8474, show primer details
   
Sequence Name 38-K018489-022-061-C04C-DL43
GenBank Accession LN502819 [GenBank]
Sequencing primer gene specific primer, DL43: 5'-ATTTCATCAATGGGATTCTCACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K018489-022-061-C04C-DL43
CTCTCTTTGCTTTGCTGCGCCTTAAACAACCAAAGTATTACTTACTTATCACTACAAACG
CCAAAATACCCCACTTATCTTACAACCAACATTACATATAAGAAGATAAGACATATCACA
TCCCAACAAATTACATTCTGTATCCTTTACTTACTCCTCTTCTAGTATATACTATCGAAG
ATTCCTGTGACCTGCAGGGCTTGGTGCTCTCTGTGAACTTATGCTCGCTGTTCTTAGAAG
TCTCCTGAACTCATGCAGGCTTATTTTCCCATCTCTGTCTATGTCTGCTTCATCCAGCAG
TGGATCTATTGATCCTCTTAACCCCGTGTGCTGAGGATTCACAAACAAATTGGGTTTCAT
CACCCACTTACCTAAACATCTACTCTACCGTACGAGAATATTGATAAGAGGTTAGTTCAG
CTTACCATTCGAAGTTCCTCAGGCGTTATGTACCCGTCTTTGTCTAGGTCGAATTTCTCA
AAAGCTGCTCTTGACCTTAGCTGCCATTTCTCTGAATCATGTTCTTCTAGTTGATGAACA
TGTAGAGCTGCTGCTACAAACTCTGTGAAGTCCACTAACCCATCAGTGTTGCTATCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
   
Sequence Name 42-K018489-022-061-C04C-8409
GenBank Accession LN504296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K018489-022-061-C04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGATAGCAACACTGATGGGTTAGTG
GACTTCACAGAGTTTGTAGCAGCAGCTCTACATGTTCATCAACTAGAAGAACATGATTCA
GAGAAATGGCAGCTAAGGTCAAGAGCAGCTTTTGAGAAATTCGACCTAGACAAAGACGGG
TACATAACGCCTGAGGAACTTCGAATGGTAAGCTGAACTAACCTCTTATCAATATTCTCG
TACGGTAGAGTAGATGTTTAGGTAAGTGGGTGATGAAACCCAATTTGTTTGTGAATCCTC
AGCACACGGGGTTAAGAGGATCAATAGATCCACTGCTGGATGAAGCAGACATAGACAGAG
ATGGGAAAATAAGCCTGCATGAGTTCAGGAGACTTCTAAGAACAGCGAGCATAAGTTCAC
AGAGAGCACCAAGCCCTGCAGGTCACAGGAATCTTCGATAGTATATACTAGAAGAGGAGT
AAGTAAAGGATACAGAATGTAATTTGTTGGGATGTGATATGTCTTATCTTCTTATATGTA
ATGTTGGTTGTAAGATAAGTGGGGTATTTTGGCGTTTGTAGTGATAAGTAAGTAATACTT
TGGTTGTTTAAGGCGCAGCAAAACAAAGAGAGAAAGTGGAGTGAGAATCC

Last Updated on 06.12.2018 16:11