Sequences from confirmation amplicon


Confirmation sequences for line 072G03 and hit At1g10430 are shown.
Line ID 072G03
Locus At1g10430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3430147
Primers used for PCR CK57 - 8474, show primer details
   
Sequence Name 18-K019980-022-072-G03C-CK57
GenBank Accession LN494118 [GenBank]
Sequencing primer gene specific primer, CK57: 5'-TAGCTGAAACAGAGAAGCAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K019980-022-072-G03C-CK57
TGCATCAAAACAGGATAGTACTTTGCTGATCAGCCAGAAACCAATGAAGAAAAGTACGAT
TCGCTCAAATATAAGCTTATTTCAAAACAAAAGCTTACACTTGAGTAATCTGCCGACTCT
CGTGATTCCCTCGTAAGATTGTAAGTCTATCCCTGTATCGCACTTTTAGTGCCACCAATA
GAGAAACTGTCTCTACTGAATAGTAGCCACGATCTGCAAAGAACAAAGCAAGACAATGAG
AAACCAACAACACTCCTTTTAAACTAAATCTAGCTGTCACCATCTTATGGTACACCAACG
AATGGTTAAAGTTCCAAGGATCTAAAGACTAGAATTCAAGGGAAGTGCAATAATGAAAGT
GAGCATCAACAAGTCTCTATATTGGGAAGTTTCAGTACCTACCATTCCAAAAAAAAACAG
AACCCACAAGTAACTCACTCTAGATAAATAAAAAGGTGGATACTTTTCCAAGAATTCACC
ATTTTGGAATGATCCAAAAACACAGATCTAAAATGAGAAAAAAAAGTAAAAATGGCTTCA
TGGCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTA
   
Sequence Name 22-K019980-022-072-G03C-8409
GenBank Accession LN495918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K019980-022-072-G03C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAGCCATGAAGC
CATTTTTACTTTTTTTTCTCATTTTAGATCTGTGTTTTTGGATCATTCCAAAATGGTGAA
TTCTTGGAAAAGTATCCACCTTTTTATTTATCTAGAGTGAGTTACTTGTGGGTTCTGTTT
TTTTTTGGAATGGTAGGTACTGAAACTTCCCAATATAGAGACTTGTTGATGCTCACTTTC
ATTATTGCACTTCCCTTGAATTCTAGTCTTTAGATCCTTGGAACTTTAACCATTCGTTGG
TGTACCATAAGATGGTGACAGCTAGATTTAGTTTAAAAGGAGTGTTGTTGGTTTCTCATT
GTCTTGCTTTGTTCTTTGCAGATCGTGGCTACTATTCAGTAGAGACAGTTTCTCTATTGG
TGGCACTAAAAGTGCGATACAGGGATAGACTTACAATCTTACGAGGGAATCACGAGAGTC
GGCAGATTACTCAAGTGTAAGCTTTTGTTTTGAAATAAGCTTATATTTGAGCGAATCGTA
CTTTTCTTCATTGGTTTCTGGCTGATCAGCAAAGTACTATCCTGTTTTGATGCATGTTTT
GCTT

Last Updated on 06.12.2018 16:11