Sequences from confirmation amplicon


Confirmation sequences for line 076F11 and hit At4g25560 are shown.
Line ID 076F11
Locus At4g25560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13052332
Primers used for PCR AB87 - 8474, show primer details
   
Sequence Name 12-K016436-022-076-F11C-8409
GenBank Accession LN491352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K016436-022-076-F11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCATAAGAAATTAA
CACATAAATTATATCCCAAATTTTCATAAAAATAGAAATATATATGTCATTTGTCATTTA
CTCTAAGCAGGCCCATTTGGTCATTTCTTAACACCAAGTATGTAGGCTCTATCTGTGGAC
GTTTGGTGATATATATGTTGTCTCAATCACCATATATAGTATTGAGACATGTTTTCTTGT
ATCAGGACAGACAGCTGACTAAAGAGAAATGGCGAAGACGAAATATGGAGAGAGACATAG
GAAAGGGTTATGGTCACCTGAAGAAGACGAGAAGCTAAGGAGCTTCATCCTCTCTTATGG
CCATTCTTGCTGGACCACTGTTCCCATCAAAGCTGGTCTCTCTTTCATCTTCTTCTTTAT
TAGTTTTTAGTTTCTCAAACTGAGTCAACTTTGATAATTGTTGTTGGGATTTAATTTGAT
AGGGTTACAAAGGAATGGGAAGAGCTGCAGATTAAGATGGATTAATTACCTAAGACCAGG
GTTAAAGAGGGATATGATTAGTGCAGAAGAAGAAGAGACTATCTTGACGTTTCATTCTTC
CTTGGGTAACAAGTAAGTGTATCTCTAAGACCTAACCCTATTGTTTTAGCAGATGCTAGT
TATAGAGAGACTAATGATTTAGGTGTAATTAGGTGGTCGCAAATAGCTAAATTCTTACCG
GGAAGAACAGACAATGAGATAAAGAACTATTGGCACTCTCATTTGAAA
   
Sequence Name 16-K016436-022-076-F11C-8601
GenBank Accession LN493208 [GenBank]
Sequencing primer gene specific primer, 8601: unknown
Sequence (A. th genome BLAST matches underlined) 
>16-K016436-022-076-F11C-8601
GGAGGGAAATAGATTTTGCATCTTGTAAGCTCTGAGACTTGAGCCATTTCTTTTTCAAAT
GAGAGTGCCAATAGTTCTTTATCTCATTGTCTGTTCTTCCCGGTAAGAATTTAGCTATTT
GCGACCACCTAATTACACCTAAATCATTAGTCTCTCTATAACTAGCATCTGCTAAAACAA
TAGGGTTAGGTCTTAGAGATACACTTACTTGTTACCCAAGGAAGAATGAAACGTCAAGAT
AGTCTCTTCTTCTTCTGCACTAATCATATCCCTCTTTAACCCTGGTCTTAGGTAATTAAT
CCATCTTAATCTGCAGCTCTTCCCATTCCTTTGTAACCCTATCAAATTAAATCCCAACAA
CAATTATCAAAGTTGACTCAGTTTGAGAAACTAAAAACTAATAAAGAAGAAGATGAAAGA
GAGACCAGCTTTGATGGGAACAGTGGTCCAGCAAGAATGGCCATAAGAGAGGATGAAGCT
CCTTAGCTTCTCGTCTTCTTCAGGTGACCATAACCCTTTCCTATGTCTCTCTCCATATTT
CGTCTTCGCCATTTCTCTTTAGTCAGCTGTCTGTCCTGATACAAGAAAACATGTCTCAAT
ACTAT

Last Updated on 06.12.2018 16:11