Sequences from confirmation amplicon


Confirmation sequences for line 080B02 and hit At5g06839 are shown.
Line ID 080B02
Locus At5g06839 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2126150
Primers used for PCR MJ06 - 8474, show primer details
   
Sequence Name 41-K017402-022-080-B02C-MJ06
GenBank Accession LN503896 [GenBank]
Sequencing primer gene specific primer, MJ06: 5'-TTAACTGGTGACAGCAAAGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017402-022-080-B02C-mj2
ACTGATCCCAAGTCCCTGACGGTATCCTACAGAACTCACCATTTGTCACCTTCCTCACTG
GTCAATGCTTTAATTTGACCTAACAAGTGATAAGTCTTGCATGCAACTGCAATTGTTGCC
AGCCTTCTATTTTTGTTTCCTTTCAATACTCTCTCATGTTCTTGTGTTTGGAATTGAAGA
TCAAGTATAATTTTTCACTTGAATTTGCATAAGGAAGAATTCATTAAAAGGTATGGAATA
CTTGTCTATATATATAATCTGCATCTTGATTAAAAACATAATAGTAAGAATCATATAACA
AAAAGAAGCCAAACAATTTTCCCTAATTGCACTGTATTTGAGTAGAGAGACGATATATAT
GATAAAGAGGTCAAGCTGGCGGCTAACTAATCAACCTATTCTCTTGTTTTTGTTTTTGGT
TTCCTTCATCAGCTGTTTTAGTATTATCCATCTTGCCGAGGACGGGCTAGCCAGAGAGAA
CTTAGAGCTTGAAGACGGTGGAAGTACTCCGTATATACACTCATATATTCAATATACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTC
   
Sequence Name 42-K017402-022-080-B02C-8409
GenBank Accession LN504287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K017402-022-080-B02C-8409
ACATGAAGCCATTTACAATTGAATATATCCTGTATATTGAATATATGAGTGTATATACGG
AGTACTTCCACCGTCTTCAAGCTCTAAGTTCTCTCTGGCTAGCCCGTCCTCGGCAAGATG
GATAATACTAAAACAACTGATGAAGGAAACCAAAAACAAAAACAAGAGAATAGGTTGATT
AGTTAGCCGCCAGCTTGACCTCTTTATCATATATATCGTCTCTCTACTCAAATACAGTGC
AATTAGGGAAAATTGTTTGGCTTCTTTTTGTTATATGATTCTTACTATTATGTTTTTAAT
CAAGATGCAGATTATATATATAGACAAGTATTCCATACCTTTTAATGAATTCTTCCTTAT
GCAAATTCAAGTGAAAAATTATACTTGATCTTCAATTCCAAACACAAGAACATGAGAGAG
TATTGAAAGGAAACAAAAATAGAAGGCTGGCAACAATTGCAGTTGCATGCAAGACTTATC
ACTTGTTAGGTCAAATTAAAGCATTGACCAGTGAGGAAGGTGACAAATGGTGAGTTCTGT
AGGATACCGTCAGGGACTTGGGATCAGTACCGTTTTGCTTTGGGTTCAACGCCCTTCTCT
CTCTCCTTTACTTTTCATTTCTTTGC

Last Updated on 06.12.2018 16:11