Sequences from confirmation amplicon


Confirmation sequences for line 086F12 and hit At5g64350 are shown.
Line ID 086F12
Locus At5g64350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25736075
Primers used for PCR 03is - 8474, show primer details
   
Sequence Name 42-K112877-0022-086-F12C-8409
GenBank Accession LN504523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112877-0022-086-F12C-8409
TTACATTGAAATAGATTACAAAATATATAGGCAAAAGCTACTCCGAGATTCGAAGCTGCA
AAGAACGCGAAACAGTGAGAGAGACAGAGAGAAGATATAGAGATGGGTGTGGAGAAGCAA
GTCATCAGACCCGGCAACGGTCCCAAACCCGCTCCGGGCCAAACCGTCACCGTCCATTGC
ACCGGATTCGGTAACTTTCCTCTGCATATTTATATCTTTGAAAATTCCATGATAGGCTAA
ATCGATCTATACAAATCTGCTTTGTTGAAGAGCTATTTGATGTGTTGTGGTAGTCGTACT
CGCTATTTGTTTGTTTTCTATGATGTGAAATAGATTTAATTAGGGTTTATGTTCTATTCT
GGGTGGTGATAGTAACACTTAGGTCATAATTAATCTGTAATTTTGCTGTTCTGCGAGATT
ATTTGTCTGCTCATCTGAAACTTCATACGAATAGATCTCTTGTTTAGATTTTCTATTTGT
AGGTTTAATTAAAGAATCTTGTTTATCTTATAATCCAGTTTCTTGTGTGGAATTGAATGG
AATTTG
   
Sequence Name 79-K112878-0022-086-F12C-03is
GenBank Accession LN513846 [GenBank]
Sequencing primer gene specific primer, 03is: 5'-ACAAATTCATTTCAATTCCACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K112878-0022-086-F12C-03is
CAGATTCTTTATTAACCTACAATAGAAAATCTAAACAAGAGATCTATTCGTATGAAGTTT
CAGATGAGCAGACAAATAATCTCGCAGAACAGCAAAATTACAGATTAATTATGACCTAAG
TGTTACTATCACCACCCAGAATAGAACATAAACCCTAATTAAATCTATTTCACATCATAG
AAAACAAACAAATAGCGAGTACGACTACCACAACACATCAAATAGCTCTTCAACAAAGCA
GATTTGTATAGATCGATTTAGCCTATCATGGAATTTTCAAAGATATAAATATGCAGAGGA
AAGTTACCGAATCCGGTGCAATGGACGGTGACGGTTTGGCCCGGAGCGGGTTTGGGACCG
TTGCCGGGTCTGATGACTTGCTTCTCCACACCCATCTCTATATCTTCTCTCTGTCTCTCT
CACTGTTTCGCGTTCTTTGCAGCTTCGAATCTCGGAGTAGCTTTTGCCTATATATTTTGT
TAATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11