Sequences from confirmation amplicon


Confirmation sequences for line 086H10 and hit K15M2 are shown.
Line ID 086H10
Locus K15M2 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5059949
Primers used for PCR 30jx - 8474, show primer details
   
Sequence Name 31-K113531-0022-086-H10C-30jx
GenBank Accession LN500242 [GenBank]
Sequencing primer gene specific primer, 30jx: 5'-GTGAATGATGACAGACCAAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113531-0022-086-H10C-30jx
TATTTTATTTCCTGATAACTTATAAATAACCGATTACTTTGATTAATAAATCATATCTCA
TGACATTTAAAAAAGATAATATCGTATTTCTCTTCTTTTATAGTCGACTACTACGTCCTT
CTTAATGCTCGAAGAGCAAACTAAATAAGATGATCCATACATACTACTTGCCTACTTGGC
CATATTGTCAACTACTTTGATGATCACAATATCATTTTGATGAGGCGTATATATATACAT
TTAATTAATATTGATGTCAAAAACTAAAACTGACGTTAATAATCAATGACATTATTGTGC
TTAATTATTGCATTTCATACAAATTTCCCCATAATTAAGAATTTAAATCTCTCGACTCTA
CATGATATGCAACCGTGGGATGACATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 06-K113530-0022-086-H10C-8409
GenBank Accession LN488894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113530-0022-086-H10C-8409
ATTTACATTGATATATGTCATCCCACGGTTGCATATCATGTAGAGTCGAGAGATTTAAAT
TCTTAATTATGGGGAAATTTGTATGAAATGCAATAATTAAGCACAATAATGTCATTGATT
ATTAACGTCAGTTTTAGTTTTTGACATCAATATTAATTAAATGTATATATATACGCCTCA
TCAAAATGATATTGTGATCATCAAAGTAGTTGACAATATGGCCAAGTAGGCAAGTAGTAT
GTATGGATCATCTTATTTAGTTTGCTCTTCGAGCATTAAGAAGGACGTAGTAGTCGACTA
TAAAAGAAGAGAAATACGATATTATCTTTTTTAAATGTCATGAGATATGATTTATTAATC
AAAGTAATCGGTTATTTATAAGTTATCATGGAAATAAAATAGTAACATGGAGGCACAATG
CTTTGCGGATATTCATGACTCTCTTTGGTCTGTCATCATTCA

Last Updated on 06.12.2018 16:11