Sequences from confirmation amplicon


Confirmation sequences for line 101D07 and hit At5g04880 are shown.
Line ID 101D07
Locus At5g04880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1419896
Primers used for PCR DL60 - 8474, show primer details
   
Sequence Name 16-K018513-022-101-D07C-DL60
GenBank Accession LN493229 [GenBank]
Sequencing primer gene specific primer, DL60: 5'-ACTTGGTCTCCTGCTTAGTCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018513-022-101-D07C-DL60
TTATCTCCTCTAGCGTTTCAGGCTTGCTTTCAGTTCGAATCTCTCTGTCCTTGGTCTCGA
CGTCAGTCCCTGGCTTCGGCATTGTGACCTGTTCAGGAGGTTTATTCTGGACTTCATCAG
ATAATCGAGATCGAAGCTCTCCCGAAACAGCTTCACTTGCAATATCTCCATTACTCATGG
AAGCCGAATCATCTCCATCTCTTGGCCGCCACATTGCAGCTGAAACAGATTGCAAGAAGC
CATTTCTGCTTGGTCCAACACAAGTATTACCCATTCCAATTGATGTTTCCCGGACATGAA
GCCATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 17-K018513-022-101-D07C-8409
GenBank Accession LN493666 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K018513-022-101-D07C-8409
CCCGGACCTGAAGCCATTTACAATTGAATATATCCTGAATGGCTTCATGTCCGGGAAACA
TCAATTGGAATGGGTAATACTTGTGTTGGACCAAGCAGAAATGGCTTCTTGCAATCTGTT
TCAGCTGCAATGTGGCGGCCAAGAGATGGAGATGATTCGGCTTCCATGAGTAATGGAGAT
ATTGCAAGTGAAGCTGTTTCGGGAGAGCTTCGATCTCGATTATCTGATGAAGTCCAGAAT
AAACCTCCTGAACAGGTCACAATGCCGAAGCCAGGGACTGACGTCGAGACCAAGGACAGA
GAGATTCGAACTGAAAGCAAGCCTGAAACGCTAGAGGAGATAAGCCTTGAGTCCAAACCA
GAGACTAAGCAGGAGACCAA

Last Updated on 06.12.2018 16:11