Sequences from confirmation amplicon


Confirmation sequences for line 106G06 and hit At5g54250 are shown.
Line ID 106G06
Locus At5g54250 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22029644
Primers used for PCR RK25 - 8474, show primer details
   
Sequence Name 27-K015894-022-106-G06C-8409
GenBank Accession LN498158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>27-K015894-022-106-G06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCAGTGAGCGACACGT
GTATGTGTCTCCTCGTCGACGGTTGGCTTGCTCTTACCGTCACTGCCTTACGCTCCATGA
CCGATCTTTTGCACCTTTGGAACATTTGGATTCAGTTCAAGATTGCTCGCCGGTGGCCTT
ATCCCGGCGGAGATAGCGACGGCGATACCAACAAAGGAGGAGGGACACGTGGCTCTACGA
GGGTTGCTCCACCTTACGTTAAGAAGAATGGGTTCTTCTTCGATCTCTTCGTCATATTAC
CATTACCTCAGGTTAGTAATTAACCAATATTTTAATATTCCTATTTTAAAGAAAACTGAT
TTAATTGTTTTGAAGTGTCTTTTATATTAGATGTAATCATTCGTATGAGCTTCTATCTTA
ATTTCTTCCTAGACTACAGAACAATCCATGGAGAAAATTTCAAAATTTATACCAAAAAAA
GAAACTCATCGTGCAAAACTATGTGAAGTTTTGGATTAGTTAAAATATTGCTTACTTATT
ATATAGTTATAATATGCCAAAATTAGATGTAGATCAAGTGGTTGTACTGAGATGGTTCCA
TTATATTAGTCGAGGCAGATTTTTCATTTTATATGCACACATAACACATTTAAATGTTCC
AAAGTTTACTATCATTTAGATAGTGACTAAACTTCAAATAAGATTACAACTACTAATACT
GTTTAAAGGCATAATCATAGTAATCAAATC
   
Sequence Name 28-K015894-022-106-G06C-RK25
GenBank Accession LN498624 [GenBank]
Sequencing primer gene specific primer, RK25: 5'-TATTATTTTTGGTCCACACACGAA-3'
Sequence (A. th genome BLAST matches underlined)
>28-K015894-022-106-G06C-rk25
GTCAGACTAAGTGAGAGTTCTTTCAGACCATATAATTATTTAGTTATAATTTTTTTTCTA
GATTACTCAATTTTAGATTTGTACTAAGATTTGATTACTATGATTATGCCTTTAAACAGT
ATTAGTAGTTGTAATCTTATTTGAAGTTTAGTCACTATCTAAATGATAGTAAACTTTGGA
ACATTTAAATGTGTTATGTGTGCATATAAAATGAAAAATCTGCCTCGACTAATATAATGG
AACCATCTCAGTACAACCACTTGATCTACATCTAATTTTGGCATATTATAACTATATAAT
AAGTAAGCAATATTTTAACTAATCCAAAACTTCACATAGTTTTGCACGATGAGTTTCTTT
TTTTGGTATAAATTTTGAAATTTTCTCCATGGATTGTTCTGTAGTCTAGGAAGAAATTAA
GATAGAAGCTCATACGAATGATTACATCTAATATAAAAGACACTTCAAAACAATTAAATC
AGTTTTCTTTAAAATAGGAATATTAAAATATTGGTTAATTACTAACCTGAGGTAATGGTA
ATATGACGAAGAGATCGAAGAAGAACCCATTCTTCTTAACGTAAGGTGGAGCAACCCTCG
TAGAGCCACGTGTCCCTCCTCCTTTGTTGGTATCGCCGTCGCTATCTCCGCCGGGATAAG
GCCACCGGCGAGCAATCTTGAACTGAATCCAAATGTTC

Last Updated on 23.10.2014 13:25