Sequences from confirmation amplicon


Confirmation sequences for line 115D02 and hit At2g20770 are shown.
Line ID 115D02
Locus At2g20770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8945914
Primers used for PCR DB12 - 8474, show primer details
   
Sequence Name 05-K029908-022-115-D02C-8409
GenBank Accession LN488227 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K029908-022-115-D02C-8409
GCCACGAGAAAGTAACATCCCTACCAAAAAAGTCTCATAGTTAACCAACAATTCAAAGCA
ACTTGTTTCTTCTATAGAACTAAGCTATTAGACAAGGGAGAAGAAAAAGAAGATACCCTG
AGGAAGCAGAAGCAGTATCACAAGCTTTGACGATTTCCAAGCAGAGGGAAAGATCGTTGG
CGTTGCCAGTAACCTGATAAGCTCTAAAGAGTAAAAACGCAGCTCCAAGAGTTCCAGAGT
AAAGAGTGAAGTCTTCAACAGTCTGTCCACTAAAACCCCAAGTTTCAATCACTACCTGAG
AGATACCAACAATAACAACTTAGTGACCATTTCAGAGTAACCTAACCAATCACAAGGAAG
AAGAGTAGATTTGCGCTTACGGTTTCTTTGAGATCCAAAGCGGACCGCTTGAGCTGCTGC
GAGAGAGACGAATACGGCATAGCAAGCAGATTCCTAAGCGTATCTCCTCCAGATACACTT
TCC
   
Sequence Name 05-K029908-022-115-D02C-DB12
GenBank Accession LN488228 [GenBank]
Sequencing primer gene specific primer, DB12: 5'-TCGTGAAGGAGAAGGAAAGTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K029908-022-115-D02C-DB12
TGCCGTATTCGTCTCTCTCGCAGCAGCTCAAGCGGTCCGCTTTGGATCTCAAAGAAACCG
TAAGCGCAAATCTACTCTTCTTCCTTGTGATTGGTTAGGTTACTCTGAAATGGTCACTAA
GTTGTTATTGTTGGTATCTCTCAGGTAGTGATTGAAACTTGGGGTTTTAGTGGACAGACT
GTTGAAGACTTCACTCTTTACTCTGGAACTCTTGGAGCTGCGTTTTTACTCTTTAGAGCT
TATCAGGTTACTGGCAACGCCAACGATCTTTCCCTCTGCTTGGAAATCGTCAAAGCTTGT
GATACTGCTTCTGCTTCCTCAGGGTATCTTCTTTTTCTTCTCCCTTGTCTAATAGCTTAG
TTCTATAGAAGAAACAAGTTGCTTTGAATTGTTGGTTAACTATGAGACTTTTTTGGTAGG
GATGTTACTTTTCTCTGTGGCCGAGCTGGTGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11