Genotyping and primer details


Genotyping and primer details for 115D02 - At2g20770
Line ID 115D02
Locus At2g20770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8945914 of line 115D02 Chr2:8945914
   
Insertion allele amplicon 8474 - DB12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DB12: 5'-TCGTGAAGGAGAAGGAAAGTGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8945417
   
wt amplicon (over InsPos) 15sq - DB12
wt amplicon start Chr2:8945417
wt amplicon end Chr2:8946417
wt amplicon size 1001 bp
Locus specific primer DB12: 5'-TCGTGAAGGAGAAGGAAAGTGTAT-3'
2nd locus specific primer 15sq: 5'-CCATCAATACGTGCATAATACCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11