Sequences from confirmation amplicon


Confirmation sequences for line 117B05 and hit At5g47120 are shown.
Line ID 117B05
Locus At5g47120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19136536
Primers used for PCR RP02 - 8474, show primer details
   
Sequence Name 52-K022410-022-117-B05C-8409
GenBank Accession LN507684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K022410-022-117-B05C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAACATGAGTGAGTATACAAATCACATTC
CCTTCTGCAAATGGAACATGACAAATGAACAAGAGGATCTTAACAATAATAAGCAGAGTA
AAGTATAACGAAAGATCTCCACAGAATCATAATCAAGTAAGTATCAACAAACTAAATCAT
GGAAAGACCCAATTAGAAACCCTAGATAAAGTACGATCGCAACGATTCGTTCAACGGAAA
CCAAATCATAGAAATTAGATGAAATATAGAAACATAGAGCGATCAATAGCACGAAAACGA
AACGAATCTCAATCTATCGACTCTAATCTAGACATCGCGGTTTGGTGTGT
   
Sequence Name 53-K022410-022-117-B05C-RP02
GenBank Accession LN508013 [GenBank]
Sequencing primer gene specific primer, RP02: 5'-CTATTCATATCACACACCAAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K022410-022-117-B05C-RP02
AGTCGATACATTGAGATTCGTTTCGTTTTCGTGCTATTGATCGCTCTATGTTTCTATATT
TCATCTAATTTCTATGATTTGGTTTCCGTTGAACGAATCGTTGCGATCGTACTTTATCTA
GGGTTTCTAATTGGGTCTTTCCATGATTTAGTTTGTTGATACTTACTTGATTATGATTCT
GTGGAGATCTTTCGTTATACTTTACTCTGCTTATTATTGTTAAGATCCTCTTGTTCATTT
GTCATGTTCCATTTGCAGAAGGGAATGTGATTTGTATACTCACTCATGTTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11