Sequences from confirmation amplicon


Confirmation sequences for line 143G10 and hit At5g59300 are shown.
Line ID 143G10
Locus At5g59300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23919788
Primers used for PCR JM03 - 8474, show primer details
   
Sequence Name 43-K022408-022-143-G10C-8409
GenBank Accession LN504697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K022408-022-143-G10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTTCTTTTTCTTCGGTTCAA
AGGGTTTCAGATAGGAATATGAAGAACTTTTGATTGAAACTCCAGAGATCTCAAAGGTGT
GAAGTTTGACGTTGATCAAAACATTTCTTGAGACTTTCTGACACAACGGCTCACTTTCTT
CTTGAACTCATCTCTCTTATCTCGCCATTCTTTCTGCGCAAGAGAAAACACACAAAATCC
ATAAGCATGTTCTGCTCTTAATTGCAAGCTATGTGCGATAAATCGATTGAAATACTCACG
GCAGCTTCAACATTTGCTGGAGACTCATCGTTAGGACCAGAAAGCATTGATATGATACTC
AACATAATACTCTCAACCTGAAAGCACCATAATTTGTTTCACTACGGCATGAACATCTGA
GACATGAAATAAATCACCTAGAGATCCCTTTATAGTGTCTGAACTATAGTAACTCTAATC
ATAGAATCTACTACTATGAAGCGAA
   
Sequence Name 44-K022408-022-143-G10C-JM03
GenBank Accession LN505045 [GenBank]
Sequencing primer gene specific primer, JM03: 5'-TGTTCATTTTTGTTCGCTTCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K022408-022-143-G10C-JM03
GATTCTATGATTAGAGTTACTATAGTTCAGACACTATAAAGGGATCTCTAGGTGATTTAT
TTCATGTCTCAGATGTTCATGCCGTAGTGAAACAAATTATGGTGCTTTCAGGTTGAGAGT
ATTATGTTGAGTATCATATCAATGCTTTCTGGTCCTAACGATGAGTCTCCAGCAAATGTT
GAAGCTGCCGTGAGTATTTCAATCGATTTATCGCACATAGCTTGCAATTAAGAGCAGAAC
ATGCTTATGGATTTTGTGTGTTTTCTCTTGCGCAGAAAGAATGGCGAGATAAGAGAGATG
AGTTCAAGAAGAAAGTGAGCCGTTGTGTCAGAAAGTCTCAAGAAATGTTTTGATCAACGT
CAAACTTCACACCTTTGAGATCTCTGGAGTTTCAATCAAAAGTTCTTCATATTCCTATCT
GAAACCCTTTGAACCGAAGAAAAAGAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11