Sequences from confirmation amplicon


Confirmation sequences for line 149H12 and hit At4g12330 are shown.
Line ID 149H12
Locus At4g12330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7318625
Primers used for PCR ZZ83 - 8474, show primer details
   
Sequence Name 27-K023373-022-149-H12C-8409
GenBank Accession LN498205 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K023373-022-149-H12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAAAGTAGACAATATAA
TACGAAAGGGAGTTGGAATGAGCTCGGAAAGTCCAATAATATCATGTAGCAATATTAGTA
AAATAATAAGAAGCTCAATTACCATTAGCAAGGCCTTGACATGGTTCATGGACATGTTTT
CATCTTCATCCTTAGCATTCAACAAAACCATCAAAAAATCTCCACCGTTCCCTTTACTTC
CCTTATCCATTCCCATCCGTTGACTTATAATCCGGTCGAACATCCGGTCCATCCTCTGAG
CCGATCTACGCACACGCTTAGCCAAACCCTGAAGGTCAAACCGGCTCAAAACCGGGAAAA
AATCAGAGACATTTGGCTTTCCCACAACATCAA
   
Sequence Name 28-K023373-022-149-H12C-ZZ83
GenBank Accession LN498674 [GenBank]
Sequencing primer gene specific primer, ZZ83: 5'-TTACGGAAATAATTGATGTTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K023373-022-149-H12C-ZZ83
CCAAATGTCTCTGATTTTTTCCCGGTTTTGAGCCGGTTTGACCTTCAGGGTTTGGCTAAG
CGTGTGCGTAGATCGGCTCAGAGGATGGACCGGATGTTCGACCGGATTATAAGTCAACGG
ATGGGAATGGATAAGGGAAGTAAAGGGAACGGTGGAGATTTTTTGATGGTTTTGTTGAAT
GCTAAGGATGAAGATGAAAACATGTCCATGAACCATGTCAAGGCCTTGCTAATGGTAATT
GAGCTTCTTATTATTTTACTAATATTGCTACATGATATTATTGGACTTTCCGAGCTCATT
CCAACTCCCTTTCGTATTATATTGTCTACTTTACAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11