Sequences from confirmation amplicon


Confirmation sequences for line 171B05 and hit At1g55530 are shown.
Line ID 171B05
Locus At1g55530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20730270
Primers used for PCR BD69 - 8474, show primer details
   
Sequence Name 03-K028341-022-171-B05C-8409
GenBank Accession LN487348 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028341-022-171-B05C-8409
CTTACAATTGAATATATCCTCAAAACGAGGTACAGACCGAGAACAATGAAGACGATGGAG
AAAATGATTTGGATTGGCAGCTTCAAGAGATTCTCAGGAGAAGGAGAAGACATTCCGCTG
CTGTTTTGCAGTTGCTTCAGGGGATACGAGCAGGGTTGTCTGTAGAATCTGAGAGTACTG
GTAATGGAGGTAATAATCCGGGACGGGTGATCTTGATCAATACCTCCAATCAGACTATCA
CGGTTCAAAACTCTGCTGATATGGATTCTGTACCTGCTGGTTCTTTAGGTGACTATTTCA
TTGGTCCTGGATTTGAAATGTTGCTTCAACGTCTAGCTGAGAATGATCCCAACAGATATG
GAACACCTCCTGCTAAAAAAGAAGCTGTCGAGGCTTTGGCTACTGTGAAAATCGAAGAGA
CTCTGCAATGTTCGGTTTGTTTAGATGATTTTGAGATTGGAACTGAGGCAAAGCTGATGC
CCTGTACGCACAAGTTTCATAGTGACTGCTTGTTACCATGGCTTGAGCTTCACAGTTCAT
GCCCGGTTTGCAGATATCAGTTACCTGCAGATGAAGCCAAGACTGATTCAGTAACAACAA
CGAGCGATAACAA
   
Sequence Name 03-K028341-022-171-B05C-BD69
GenBank Accession LN487349 [GenBank]
Sequencing primer gene specific primer, BD69: 5'-ACTACTGCTTCCATTGTTATCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028341-022-171-B05C-BD69
AGCCAGGTAACAAGCAGTCACTATGAAACTTGTGCGTACAGGGCATCAGCTTTGCCTCAG
TTCCAATCTCAAAATCATCTAAACAAACCGAACATTGCAGAGTCTCTTCGATTTTCACAG
TAGCCAAAGCCTCGACAGCTTCTTTTTTAGCAGGAGGTGTTCCATATCTGTTGGGATCAT
TCTCAGCTAGACGTTGAAGCAACATTTCAAATCCAGGACCAATGAAATAGTCACCTAAAG
AACCAGCAGGTACAGAATCCATATCAGCAGAGTTTTGAACCGTGATAGTCTGATTGGAGG
TATTGATCAAGATCACCCGTCCCGGATTATTACCTCCATTACCAGTACTCTCAGATTCTA
CAGACAACCCTGCTCGTATCCCCTGAAGCAACTGCAAAACAGCAGCGGAATGTCTTCTCC
TTCTCCTGAGAATCTCTTGAAGCTGCCAATCCAAATCATTTTCTCCATCGTCTTCATTGT
TCTCGGTCTGTACCTCGTTTTGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11