Sequences from confirmation amplicon


Confirmation sequences for line 181H03 and hit At5g13790 are shown.
Line ID 181H03
Locus At5g13790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4450887
Primers used for PCR MY01 - 8474, show primer details
   
Sequence Name 27-K020888-022-181-H03C-8409
GenBank Accession LN498188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K020888-022-181-H03C-8409
GATTTCCCGGAGATTTCGCATAATCTCCATATTGACCATCATTCTCATCTAAACCCATTT
TGGAATACATTGAACCTTTCCTCTTCTTCTTCTTCCTTCTACCTTCTTCTCTCTGTTCAA
TTTTGGGGGAAAATGGGTCGTGGAAAAATCGAGATAAAGAGGATCGAGAATGCGAATAGC
AGACAAGTCACTTTTTCCAAGAGGCGTTCTGGGTTACTTAAGAAAGCTCGTGAGCTCTCT
GTTCTTTGTGATGCTGAAGTTGCTGTCATCGTCTTCTCTAAGTCTGGCAAGCTCTTCGAG
TACTCCAGTACTGGGTAACACTTATTTCTTTTTGATTCAATTTTGTTTTTGCATGTCTTG
TCTTGTTGTGATTAGAATCGATTTCGGGAACTGTAATTGATTTTTGTTTTTGCATGTTTG
TTAAGATTAAAAGTTTTCTGATTGAGCTGAAGAGAGTCCTAATTTTGAATTCTCATTTGA
TTTTAGAATGAAGCAAACACTTTCCAGATACGGTAATCACCAGAGTT
   
Sequence Name 28-K020888-022-181-H03C-MY01
GenBank Accession LN498657 [GenBank]
Sequencing primer gene specific primer, MY01: 5'-AGAAGCTGAAGAACTCTGGTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K020888-022-181-H03C-MY01
CGTATCTGGAAAGTGTTTGCTTCATTCTAAAATCAAATGAGAATTCAAAATTAGGACTCT
CTTCAGCTCAATCAGAAAACTTTTAATCTTAACAAACATGCAAAAACAAAAATCAATTAC
AGTTCCCGAAATCGATTCTAATCACAACAAGACAAGACATGCAAAAACAAAATTGAATCA
AAAAGAAATAAGTGTTACCCAGTACTGGAGTACTCGAAGAGCTTGCCAGACTTAGAGAAG
ACGATGACAGCAACTTCAGCATCACAAAGAACAGAGAGCTCACGAGCTTTCTTAAGTAAC
CCAGAACGCCTCTTGGAAAAAGTGACTTGTCTGCTATTCGCATTCTCGATCCTCTTTATC
TCGATTTTTCCACGACCCATTTTCCCCCAAAATTGAACAGAGAGAAGAAGGTAGAAGGAA
GAAGAAGAAGAGGAAAGGTTCAATGTATTCCAAAATGGGTTTAGATGAGAATGATGGTCA
ATATGGAGATTATGCGAAATCTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC

Last Updated on 06.12.2018 16:11