Sequences from confirmation amplicon


Confirmation sequences for line 200A02 and hit At3g52750 are shown.
Line ID 200A02
Locus At3g52750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19552236
Primers used for PCR 23xy - 8474, show primer details
   
Sequence Name 35-K119596-0022-200-A02C-23xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 23xy: 5'-GCTAGAAAAGCTTAGGCACTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K119596-0022-200-A02C-23xy
AACATGTAAATGTCCGCACCGTTTATCATAAAAGTATTCTATGATCGAAGACATAAACCT
CACTGCTTTAACCAGATGGAGATAAAAGAGAAACTGGTAAACACAAAGAGAAACTGCAAC
CCACCAAAATTCAATAAAACCAACATGATATTGCAAACGGCGATTCTGACCCGTAAAACT
TCTCATTTGTAATCTATATCTTTATATAGAAATAAGTATAAATAAAAGTTTGTTACTAAG
AAAAAGTGAAAGTAATAGTTATAGACATACTGTGACAAAGACCATATCTGAACCATAAAG
TGCTTCTTGAATAGCTTCTTTGCTTTCTGTAGCAGCATTCATCCCAATCTCTGGATTACC
TCCAGCACCTAAACCCCTAGTCAACTCCTTACCAATTTGTAACCTATTGTCTGGAAAAAC
CGGAGATATTCTCATTGCTTGGATATCGGTATTCACAATCCAAAACTCCACACCAATCAT
CTCACTCTCAATCATACGATTCACAGCGTTTGAACCACCACCTCCAACGCCGATAACTTT
AATCCTAGCCTCATTGTAAGTGTTTGGAGTAGATAACTCATCCAAATCCTCAGTAATGGG
AACACTGGAAGTTTCTTTTCTTGGGTTGAGCATGGATATCTCAGGGTGGAGATTCAAGAA
AGAGTCTTGGCTTTGGAAATGGCTTGTACTATCTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K119595-0022-200-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K119595-0022-200-A02C-8409
CTTTCATTGAATATATCCTGAGATAGTACAAGCCATTTCCAAAGCCAAGACTCTTTCTTG
AATCTCCACCCTGAGATATCCATGCTCAACCCAAGAAAAGAAACTTCCAGTGTTCCCATT
ACTGAGGATTTGGATGAGTTATCTACTCCAAACACTTACAATGAGGCTAGGATTAAAGTT
ATCGGCGTTGGAGGTGGTGGTTCAAACGCTGTGAATCGTATGATTGAGAGTGAGATGATT
GGTGTGGAGTTTTGGATTGTGAATACCGATATCCAAGCAATGAGAATATCTCCGGTTTTT
CCAGACAATAGGTTACAAATTGGTAAGGAGTTGACTAGGGGTTTAGGTGCTGGAGGTAAT
CCAGAGATTGGGATGAATGCTGCTACAGAAAGCAAAGAAGCTATTCAAGAAGCACTTTAT
GGTTCAGATATGGTCTTTGTCACAGTATGTCTATAACTATTACTTTCACTTTTTCTTAGT
AACAAACTTTTATTTATACTTATTTCTATATAAAGATATAGATTACAAATGAGAAGTTTT
ACGGGTCAGAATCGCCGTTTGCAATATCATGTTGGTTTTATTGAATTTTGGTGGGTTGCA
GTTTCTCTTTGTGTTTACCAGTTTCTCTTTTATCTCCATCTGGTTAAAGCAGTGAGGTTT
ATGTCTTCGATCATAGAATACTTTCCTGTTTAAAGTACAACCAGTTATGGGTTATTGTGT
GAATTTGCTGCCTCCATAGATTGACTGTAAGATTAGATCTTTAAGTTCAGTGCCTAACTT
TTTTCTA

Last Updated on 06.12.2018 16:11