Sequences from confirmation amplicon


Confirmation sequences for line 208G12 and hit At5g38100 are shown.
Line ID 208G12
Locus At5g38100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15199751
Primers used for PCR MJ34 - 8474, show primer details
   
Sequence Name 13-K029909-022-208-G12C-8409
GenBank Accession LN491908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K029909-022-208-G12C-8409
CATTACAATTGAATATATCATGATTCTTCTTCATTATATCATTATTGTATTTATTTTCGT
TTAAGTACTATACAATACACCANNCTTGCTTTTTCCGCATTTCAAAATCAATTGGGTGAT
TGGAGAGTTTTTTAGCAAGTCGATAGAATAGCTCATCAACCACACCGTCTCCAAAATGTT
TTTCTATGATTGTAGTGAGAAAAGCTCGAAACGTGGAAGTGATGAAGTCGTTGGTTAATG
GCTTACCCTCCAATGGATGACTTGTGGTCTCCATCAGCTCAATCGTAAAGCTTCCATTTT
TCTCAATCTCTCCCTTTAATTCACTAAATTGAGGAAAATATACTGGCAAACTAAATAAAT
CGATCTTTTCCTCGCTTGTTATTCCCTAAAAAAAAGATAACTAATGATTACTAAAAACAA
TCATGCTTTTTAAATATACTAAAAAGATAATTATCTTACCGATTTAGCCATATCCAT
   
Sequence Name 13-K029909-022-208-G12C-MJ34
GenBank Accession LN491909 [GenBank]
Sequencing primer gene specific primer, MJ34: 5'-GGTGATTGTCTTATGGATATGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K029909-022-208-G12C-MJ34
GCATGATTGTTTTACGTAATCATTAGTTATCTTTTTTTAGGGAATAACAAGCGAGGAAAA
GATCGATTTATTTAGTTTGCCAGTATATTTTCCTCAATTTAGTGAATTAAAGGGAGAGAT
TGAGAAAAATGGAAGCTTTACGATTGAGCTGATGGAGACCACAAGTCATCCATTGGAGGG
TAAGCCATTAACCAACGACTTCATCACTTCCACGTTTCGAGCTTTTCTCACTACAATCAT
AGAAAAACATTTTGGAGACGGTGTGGTTGATGAGCTATTCTATCGACTTGCTAAAAAACT
CTCCAATCACCCAATTGATTTTGAAATGCGGAAAAAGCAAGTGGTGTATTGTATAGTACT
TAAACGAAAATAAATACAATAATGATATAATGAAGAAGAATCATGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11