Sequences from confirmation amplicon


Confirmation sequences for line 219F01 and hit At2g27220 are shown.
Line ID 219F01
Locus At2g27220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11638273
Primers used for PCR JU05 - 8474, show primer details
   
Sequence Name 19-K019822-022-219-F01C-JU05
GenBank Accession LN494565 [GenBank]
Sequencing primer gene specific primer, JU05: 5'-AATTTGACGAGCAGCTTAAGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K019822-022-219-F01C-JU05
ATGGCACATCACCATTCAAATGCTTGGAGACCGCAGAGAGGTTTGCCCGAAAAAGCTGTT
TCCGTTCTACGCTCTTGGCTCTTTGAGCATTTTCTTCATCCGAAAGTAACGTTTTCTTCT
ATCTTTAATTCTTGTTACGATTATTGTCGATATATCAGAATGATGGGACCTTGAAGCAAA
GGGAACTAACTAAGGATA
   
Sequence Name 20-K019822-022-219-F01C-8409
GenBank Accession LN495020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K019822-022-219-F01C-8409
TGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAACTTCTGATAATCGAAATCAAT
TACCTGACTCTTAGTAAGGCCAGTCTGTTTGGCAAGCATTACCTTATCCAAATCCCTCGG
ATATCTATACAAACCAAAACAAGACACAACTAAATCATTAGCATGCATGTCTATACAAAA
CTAAGTAATGATCCTAGCTTCCATATCCTTAGTTAGTTCCCTTTGCTTCAAGGTCCCATC
ATTCTGATATATCGACAATAATCGTAACAAGAATTAAAGATAGAAGAAAACGTTACTTAC
GGATGAAGAAAATGCTCAAAGAGCCAAGAGCGTAGAACGGAAACAGCTTTTTCGGGCAAA
CCTCTCTGCGGTCTCCAAGCATTTGAATGGTGATGTGCCATCTTTCCCAACTTCTTAAGC
TGCTCGTCAAA

Last Updated on 06.12.2018 16:11