Sequences from confirmation amplicon


Confirmation sequences for line 224F11 and hit At1g11260 are shown.
Line ID 224F11
Locus At1g11260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3780192
Primers used for PCR RK14 - 8474, show primer details
   
Sequence Name 45-K015847-022-224-F11C-8409
GenBank Accession LN505356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K015847-022-224-F11C-8409
ATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATAATTTGTAGAAATTACAAATC
TTTTTTCTTTCTTTCTTAAAACTCTCATTAAAAACAATGATAAATCAAACATGCTTCGTT
CCAGCTTGGTTACTGTTCTTGCCCATCTCAAGCGCATTCCCATACTCACCATCCTCCACA
AACCTTGACCAATACCAGTGTGACCTCCACACTTGACCCATCTCCTCTATCGGAATCCCT
TTCGTCTCCGGCAAGAAAATGTATACAAAGATCGACATCACCACCACGAAAAAGGCGAAA
ACAAGGAATAACCCAAACTTCAAATGACAAAGCATCGTCAAGAAGATTTGCGCGATAATG
AACGTGAAGATCATGTTCACGGACACGGTGATACTCTGCGCCGCCGACCTTATCTCCAAC
GGGAAGATTTCACTCGGTACTAACCACCCTAGTGGGCCCCACGACCACGCAAAACCCGCC
ACATAGATGCAAATGAACGTTACAACCACTATAGCATACCACTTTGGTAGCTCACCAGGG
GTCCCGTCTACCCCAAACTTGGCCCCTATGCAAGCTGCAACCACAGCCTACAATTACACA
GAGAGT
   
Sequence Name 46-K015847-022-224-F11C-RK14
GenBank Accession LN505703 [GenBank]
Sequencing primer gene specific primer, RK14: 5'-GATGTGTTGGTTCACTCTCTGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K015847-022-224-F11C-rk14
GCTTGCATAGGGGCCAAGTTTGGGGTAGACGGGACCCCTGGTGAGCTACCAAAGTGGTAT
GCTATAGTGGTTGTAACGTTCATTTGCATCTATGTGGCGGGTTTTGCGTGGTCGTGGGGC
CCACTAGGGTGGTTAGTACCGAGTGAAATCTTCCCGTTGGAGATAAGGTCGGCGGCGCAG
AGTATCACCGTGTCCGTGAACATGATCTTCACGTTCATTATCGCGCAAATCTTCTTGACG
ATGCTTTGTCATTTGAAGTTTGGGTTATTCCTTGTTTTCGCCTTTTTCGTGGTGGTGATG
TCGATCTTTGTATACATTTTCTTGCCGGAGACGAAAGGGATTCCGATAGAGGAGATGGGT
CAAGTGTGGAGGTCACACTGGTATTGGTCAAGGTTTGTGGAGGATGGTGAGTATGGGAAT
GCGCTTGAGATGGGCAAGAACAGTAACCAAGCTGGAACGAAGCATGTTTGATTTATCATT
GTTTTTAATGAGAGTTTTAAGAAAGAAAGAAAAAAGATTTGTAATTTCTACAAATTATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA

Last Updated on 06.12.2018 16:11