Sequences from confirmation amplicon


Confirmation sequences for line 225C06 and hit At4g01280 are shown.
Line ID 225C06
Locus At4g01280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:535492
Primers used for PCR GC07 - 8474, show primer details
   
Sequence Name 21-K018967-022-225-C06C-GC07
GenBank Accession LN495465 [GenBank]
Sequencing primer gene specific primer, GC07: 5'-ACACTAGTTTAAGGCGTCAGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K018967-022-225-C06C-GC07
CTGGGTACTACAGCAACGAGACCCTAACACGTGTAAAGGAAACAATATTATGCTCCTGAA
ATTACAATTCTACCCCTCTGTTCTTCAGCCACAAAAAAAAACTGGCGAAGTCAAAATCTT
TTGACAGTGAAAGGACCTTTGAAGAAGAAGAATCGCGATTTCGAGCTCTATGGTGTCCGT
AAACCCTAGACCTAAGGGTTTTCCAGTTTTCGATTCCTCGAATATGAGTTTACCAAGCTC
CGATGGATTTGGTTCGATTCCGGCCACGGGACGGACCAGTACGGTGTCGTTTTCTGAGGA
TCCGACGACGAAGATTCGGAAGCCGTACACAATCAAGAAGTCGAGAGAGAATTGGACAGA
TCAAGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 22-K018967-022-225-C06C-8409
GenBank Accession LN495913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K018967-022-225-C06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTCTTGATCTGT
CCAATTCTCTCTCGACTTCTTGATTGTGTACGGCTTCCGAATCTTCGTCGTCGGATCCTC
AGAAAACGACACCGTACTGGTCCGTCCCGTGGCCGGAATCGAACCAAATCCATCGGAGCT
TGGTAAACTCATATTCGAGGAATCGAAAACTGGAAAACCCTTAGGTCTAGGGTTTACGGA
CACCATAGAGCTCGAAATCGCGATTCTTCTTCTTCAAAGGTCCTTTCACTGTCAAAAGAT
TTTGACTTCGCCAGTTTTTTTTTGTGGCTGAAGAACAGAGGGGTAGAATTGTAATTTCAG
GAGCATAATATTGTTTCCTTTACACGTGTTAGGGTCTCGTTGCTCTAGTACCCAGACTAT
ACCACTGACGCCTTAAACTAGT

Last Updated on 06.12.2018 16:11