Genotyping and primer details


Genotyping and primer details for 225C06 - At4g01280
Line ID 225C06
Locus At4g01280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:535492 of line 225C06 Chr4:535492
   
Insertion allele amplicon 8474 - GC07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GC07: 5'-ACACTAGTTTAAGGCGTCAGTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:535090
   
wt amplicon (over InsPos) 09rd - GC07
wt amplicon start Chr4:535090
wt amplicon end Chr4:535798
wt amplicon size 709 bp
Locus specific primer GC07: 5'-ACACTAGTTTAAGGCGTCAGTGGT-3'
2nd locus specific primer 09rd: 5'-TAGCACCACTCTTCTGAACTTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11