Sequences from confirmation amplicon


Confirmation sequences for line 227H02 and hit At3g16940 are shown.
Line ID 227H02
Locus At3g16940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5783689
Primers used for PCR 21ii - 8474, show primer details
   
Sequence Name 69-K112621-0022-227-H02C-21ii
GenBank Accession LN512234 [GenBank]
Sequencing primer gene specific primer, 21ii: 5'-CTTTTCCATTTTGTAATCCAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K112621-0022-227-H02C-21ii
ATACCTAGTTTTCTCGGAAGATAGGCCCAGCAGGTGAAACATCGGTTATGTTAAACACTT
GCTCAGGTATGTTAGAGTGAGAATGAAATACAGCTTGAGGAGCTAATGGATCCTGCCTAG
GCATAACCATGGGTTCAAAGGAAGGATCCTCCAGTGAGCCATTAGATTCGCTGATAAATG
AATTCATCCACCTTCCAAAACTCTCTCGACTTTGTGGACCATCGTTAAGCAAAGCATCAA
GAGATCCATCAGCCACTGTCGCATTCCCATGCTCTGCAGTGCCATTTGCAGCATTTTGGA
GCGGTTCTGTGAAATATGAAAGATTATCTGATCACAGATAAAAAGAAAGATTTATTTTCT
TTAAATCTACATGCATTGGGAATTGTCAGCATTTGCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 53-K112620-0022-227-H02C-8409
GenBank Accession LN508200 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112620-0022-227-H02C-8409
TTGAATATATCCTGCAAATGCTGACAATTCCCAATGCATGTAGATTTCAATAAAATAAAT
CTTTCTTTTTATCTGTGATCAGATAATCTTTCATATTTCACAGAACCGCTCCAAAATGCT
GCAAATGGCACTGCAGAGCATGGGAATGCGACAGTGGCTGATGGATCTCTTGATGCTTTG
CTTAACGATGGTCCACAAAGTCGAGAGAGTTTTGGAAGGTGGATGAATTCATTTATCAGC
GAATCTAATGGCTCACTGGAGGATCCTTCCTTTGAACCCATGGTTATGCCTAGGCAGGAT
CCATTAGCTCCTCAAGCTGTATTTCATTCTCACTCTAACATACCTGAGCAAGTGTTTAAC
ATAACCGATGTTTCACCTGCTTGGGCCTATTCTTCCGAGAAAACTAAGGTATGTGCTATC
ATATGAATAAAATAATTGGTTTGGATTACAAATTGGAAAA

Last Updated on 06.12.2018 16:11