Sequences from confirmation amplicon


Confirmation sequences for line 242H01 and hit At3g30180 are shown.
Line ID 242H01
Locus At3g30180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11811819
Primers used for PCR MS53 - 8474, show primer details
   
Sequence Name 10-K017193-022-242-H01C-MS53
GenBank Accession LN490427 [GenBank]
Sequencing primer gene specific primer, MS53: 5'-AATAGTCTCAATGGACGCAGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K017193-022-242-H01C-MS53
AGATACATTCTAATGAATGAATCGAAAGGACTTGTTGCCGGTTACCCGCAATCTATGCTT
GATATTCTAGGGACATGCAACATAGCTGCGGTTCATGGCCCGAGCCACCGGCTAATGAGA
GGCTCGTTGCTTTCTTTAATAAGCCCAACCATGATGAAAGACCATCTCTTGCCTAAGATT
GATGATTTCATGAGAAACTATCTTTGTGGTTGGGATGATCTTGAGACAGTTGATATCCAA
GAAAAGACCAAACATGTACGTTTTTTAAGCTTTTCTCTCTCTTTCTCTCTTTGTTTTTTA
GACATTGAATCTGAATCTCGGGTCTATATCCTTTATTGGGGATGTAATGTTTTTAATATG
TCAGAGCAATTTATTGTTTTTGTTAAAAAGAAAATTAATTACTATCATTCTTTTTGACAA
AAGAAATTTGTTCGTCCTCACTTTAATTAATTTTGTTGGTCTATTAGATTCATATTATAG
CCAAACTATTTTTTTGTTGATTTTAGGTAAAAGGTATATATTATTCTAAATTCTCTCAGG
GAGTTAGTTTTTATTTTGTTTTGGAGGGGTAGAATCGTAATTTGACGATAATTGTTAATA
ATTGTTAATTATTTGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATG
   
Sequence Name 14-K017193-022-242-H01C-8409
GenBank Accession LN492283 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017193-022-242-H01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCAAATAATTAACAATT
ATTAACAATTATCGTCAAATTACGATTCTACCCCTCCAAAACAAAATAAAAACTAACTCC
CTGAGAGAATTTAGAATAATATATACCTTTTACCTAAAATCAACAAAAAAATAGTTTGGC
TATAATATGAATCTAATAGACCAACAAAATTAATTAAAGTGAGGACGAACAAATTTCTTT
TGTCAAAAAGAATGATAGTAATTAATTTTCTTTTTAACAAAAACAATAAATTGCTCTGAC
ATATTAAAAACATTACATCCCCAATAAAGGATATAGACCCGAGATTCAGATTCAATGTCT
AAAAAACAAAGAGAGAAAGAGAGAGAAAAGCTTAAAAAACGTACATGTTTGGTCTTTTCT
TGGATATCAACTGTCTCAAGATCATCCCAACCACAAAGATAGTTTCTCATGAAATCATCA
ATCTTAGGCAAGAGATGGTCTTTCATCATGGTTGGGCTTATTAAAGAAAGCAACGAGCCT
CTCATTAGCCGGTGGCTCGGGCCATGAACCGCAGCTATGTTGCATGTCCCTAGAATATCA
AGCATAGATTGCGGGTAACCGGCAACAAGTCCTTTCGATTCATTCATTAGAATGTATCTG
TTTAACTCTGCGTCCA

Last Updated on 06.12.2018 16:11