Genotyping and primer details


Genotyping and primer details for 242H01 - At3g30180
Line ID 242H01
Locus At3g30180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:11811819 of line 242H01 Chr3:11811819
   
Insertion allele amplicon 8474 - MS53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MS53: 5'-AATAGTCTCAATGGACGCAGAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:11811193
   
wt amplicon (over InsPos) 07gy - MS53
wt amplicon start Chr3:11811193
wt amplicon end Chr3:11812194
wt amplicon size 1002 bp
Locus specific primer MS53: 5'-AATAGTCTCAATGGACGCAGAGTT-3'
2nd locus specific primer 07gy: 5'-TCTCCAGACTCTTTTCTTTCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11