Sequences from confirmation amplicon


Confirmation sequences for line 254B10 and hit F15O5 are shown.
Line ID 254B10
Locus F15O5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25996887
Primers used for PCR KN40 - 8474, show primer details
   
Sequence Name 58-K103246-0022-254-B10C-KN40
GenBank Accession LN509676 [GenBank]
Sequencing primer gene specific primer, KN40: 5'-ACGCGATTGACATCAATAATCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K103246-0022-254-B10C-KN40
CGTGACTCCTATTTTTTATTTTTATTTCTCTCTAGAGTTTTACCTGTCAAATTACCTATC
TAGTTCTTTTTTTTCTTTTTCTTTTACGACTACTAGCTAGAGAGACATTTGCACATACNG
TAAAGGAATTTATTTTATGTAGGGGTTCAAACACTTGGGAAGAAGAGGATTTACAGACTT
TTTTAACATGAAATTATTGGATGACTCTTCTTCCCCTAAGCTAAATTTACAGACTTTTTG
TTGTTTGGAAAAAGTTCTTCATATAAAAAACAATATGAACAGTAACCTGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGA
   
Sequence Name 10-K103247-0022-254-B10C-8409
GenBank Accession LN490611 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K103247-0022-254-B10C-8409
ATTGTTTTTTAATGAAGAACTTTTTCCAACAACAAAAAGTCTGTAAATTTAGCTTAGGGG
AAGAAGAGTCATCCAATAATTTCATGTTAAAAAAGTCTGTAAATCCTCTTCTTCCCAAGT
GTTTGAACCCCTACATAAAATAAATTCCTTTACTGTATGTGCAAATGTCTCTCTAGCTAG
TAGTCGTAAAAGAAAAAGAAAAAAAAGAACTAGATAGGTAATTTGACAGTGTAAAACTCT
AGAGAGAAATAAAAAATAAAAAAATTAGGAAGATCAACGGAAATTGCGTTGACTATGATT
ATTGATGTCAATCGCG

Last Updated on 06.12.2018 16:11