Sequences from confirmation amplicon


Confirmation sequences for line 273E01 and hit T7M7 are shown.
Line ID 273E01
Locus T7M7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16797937
Primers used for PCR PH30 - 8474, show primer details
   
Sequence Name 53-K017546-022-273-E01C-PH30
GenBank Accession LN507991 [GenBank]
Sequencing primer gene specific primer, PH30: 5'-GAGGAGAGGTCTTAGGGTTTGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K017546-022-273-E01C-PH30
GAAGGAGAAGAAGCGGCGGAGACGGAGGAGGAAGAGGAAGAGACGGAGGAAGGAGACGAA
GGGGTTAAGTTGAGCTGAGCACGTGACCCGTATAGGTAAACGGCAGCCCGGTCGTAGGCA
CGTGCGGCGTCTTCGGCGGTTGCGAAAGTACCAAGCCACTTGCGAGTGCGCTTACGTGGC
TCTCGGATCTCGGCGACCCATTTGCCCCAGCTTCTTTGTCGAACGCCACGGTAACGGAAC
TTGGAGTTGTCCGGACCACCTTTGCCTTTGCGTTTGCGTTGAGCGGAGGAAGTTGATGAG
TCGGTGGTGGAATCGGATTGAGGATTATTATGGGTTAGGGTTTGGTTATTATCTTCCANA
TGGTTGTGTTGATGTTGGGAAGCTAAAGGGCCCCTCCATGAAGAAGAAGAAGAACAAC
   
Sequence Name 57-K017546-022-273-E01C-8409
GenBank Accession LN509266 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K017546-022-273-E01C-8409
TTTCCCGGACATGAAGCCATTTACATGATTTGCTATAGTTACCCAAAAGAAAGAAAGCAA
TTAAATGTTTTTATAATATCCTTGATTACATTCCAATGTGTAACAAGTAACTTATGACAA
CACAAATTTCTTCTTTGGTAGAAAACAAACGGGTAAGTAAAAAGAAAAGAAAAAAAGAAG
TGAGTGAGAAGAGAGTGTAAGTATAAATAAAAGAAGCACCGCCCTAATCGACCATTCACA
ACGATGACATTCAAACACTCTTCAGTTTCCCTTCCTTCTTGATTCGTCCTCTCCACTATT
TTTCTCAATTTCTTTAATCTCTCTCTTTCTCTCTCTACTTCCTCTTCCTCTTCTTCTTCT
TCTTCTTCTTCATCTATGGACCCTTTAGCTTCCCAACATCAACACAACCATCTGGAAGAT
AATAACCAAACCCTAACCCATAATAATCCTCAATCCGATTCCACCACCGACTCATCAACT
TCCTCCGCTCAACGCAAACGCAAAGGCAAAGGTGGTCCGGACAACTCCAAGTTCCGTTAC
CGTGGCGTTCGACAAAGAAGCTGGGGCAAATGGGTCGCCGAGATCCGAGAGCCACGTAAG
CGCACTCGCAAGTGGCTTGGTACTTTCGCAACCGCCGAAGACGCCGCACGTGCCTACGAC
CGGGCTGCCGTTTACCTATACGGGTCCCGTGCTCAGCTCAACTTA

Last Updated on 06.12.2018 16:11