Sequences from confirmation amplicon


Confirmation sequences for line 274A06 and hit At5g58440 are shown.
Line ID 274A06
Locus At5g58440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23626229
Primers used for PCR CD11 - 8474, show primer details
   
Sequence Name 13-K029488-022-274-A06C-8409
GenBank Accession LN491905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K029488-022-274-A06C-8409
GCCATTTACATGAAGCTTGAAGAAGAAGGAGATCTCGATAGTGAATCTGAGAATTGACTA
TGAAGGCTATTATCCTCGGTACCGTTAATCTCGCTATCTGAGTTCTCATCAAAGGGACTG
AAGATAACATCTGCATACGACGGAGGCTCGATGTAGGAGTTATCGCTGCTGATCGGCTTA
GAGCTGAAGCTGCTGCGGAAATCTTCGTAGGAGGATGGCGCAAGGAGTGGATCAGAATCA
GCTGGAATCATAACGGTCGGTGGCGGCGAGAGCGGGTCACGGACGTTGGAGAGTGTTGAC
ATGGCGCTACGGTAACCAGAGTTCGATGTATCACCGTTGACATCGCTTATCTTCAGAGGA
TGGTCACCACCATCTACTCCTAGGTCGAGATTCTCCATATCATCCCTTTGCGCATTGAGA
TTAGTTTCTTCGAATCCATCGGCATTCTCTGAGCCCATCATTTCGACTTCTCTGAAAGTA
ACG
   
Sequence Name 14-K029488-022-274-A06C-CD11
GenBank Accession LN492376 [GenBank]
Sequencing primer gene specific primer, CD11: 5'-TCGTTACTCCAGAGAAGTCGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K029488-022-274-A06C-CD11
ATCTCAATGCGCAAAGGGATGATATGGAGAATCTCGACCTAGGAGTAGATGGTGGTGACC
ATCCTCTGAAGATAAGCGATGTCAACGGTGATACATCGAACTCTGGTTACCGTAGCGCCA
TGTCAACACTCTCCAACGTCCGTGACCCGCTCTCGCCGCCACCGACCGTTATGATTCCAG
CTGATTCTGATCCACTCCTTGCGCCATCCTCCTACGAAGATTTCCGCAGCAGCTTCAGCT
CTAAGCCGATCAGCAGCGATAACTCCTACATCGAGCCTCCGTCGTATGCAGATGTTATCT
TCAGTCCCTTTGATGAGAACTCAGATAGCGAGATTAACGGTACCGAGGATAATAGCCTTC
ATAGTCAATTCTCAGATTCACTATCGAGATCTCCTTCTTCTTCAAGCTTCATGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11