Genotyping and primer details


Genotyping and primer details for 274A06 - At5g58440
Line ID 274A06
Locus At5g58440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23626229 of line 274A06 Chr5:23626229
   
Insertion allele amplicon 8474 - CD11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD11: 5'-TCGTTACTCCAGAGAAGTCGAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23626698
   
wt amplicon (over InsPos) 27rd - CD11
wt amplicon start Chr5:23625885
wt amplicon end Chr5:23626698
wt amplicon size 814 bp
Locus specific primer CD11: 5'-TCGTTACTCCAGAGAAGTCGAAAT-3'
2nd locus specific primer 27rd: 5'-ACTGTTCCTAATAACAGGGTGTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11