Sequences from confirmation amplicon


Confirmation sequences for line 284H11 and hit At5g46830 are shown.
Line ID 284H11
Locus At5g46830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19003470
Primers used for PCR MH09 - 8474, show primer details
   
Sequence Name 04-K017192-022-284-H11C-MH09
GenBank Accession LN487712 [GenBank]
Sequencing primer gene specific primer, MH09: 5'-GTTATTCGGGAGCTTAACTTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K017192-022-284-H11C-MH09
TAGATTTCCCGGAATCTCCGGCGAACGTTTCCGGTGGTTGAAGACGACGTCAGTGATGAC
GATGACGTGGAAGTGACGGATATGGAGTGGTTCTTCTTGGTTTCCATGACGTGGAGTTTT
GGTAACGGATCCGGGTTAGCGGGTAAGGCGTTTGCTAGCTATAACCCGGTTTTGGTTACC
GGTTCGGATCTGATTTACGGGTCGGGTTGTGATCGGGCTAAACAAGGAGGGGATGTAGGG
TTACAGACCATCTTGTGTATTCCTTCACATAACGGAGTGTTGGAGCTTGCATCCACGGAG
GAGATCCGACCAAATTCGGATCTTTTTAATAGGATCCGGTTTCTTTTTGGAGGATCCAAA
TATTTTTCTGGAGCCCCGAATTCGAACTCGGAGCTTTTCCCATTTCAGTTAGAGAGCAGT
TGTTCTTAATCCTATTCCGACAACCCGAATATTCTTACCGCGATTTTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACAT
   
Sequence Name 08-K017192-022-284-H11C-8409
GenBank Accession LN489505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K017192-022-284-H11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAATCGCGGTAAGA
ATATTCGGGTTGTCGGAATAGGATTAAGAACAACTGCTCTCTAACTGAAATGGGAAAAGC
TCCGAGTTCGAATTCGGGGCTCCAGAAAAATATTTGGATCCTCCAAAAAGAAACCGGATC
CTATTAAAAAGATCCGAATTTGGTCGGATCTCCTCCGTGGATGCAAGCTCCAACACTCCG
TTATGTGAAGGAATACACAAGATGGTCTGTAACCCTACATCCCCTCCTTGTTTAGCCCGA
TCACAACCCGACCCGTAAATCAGATCCGAACCGGTAACCAAAACCGGGTTATAGCTAGCA
AACGCCTTACCCGCTAACCCGGATCCGTTACCAAAACTCCACGTCATGGAAACCAAGAAG
AACCACTCCATATCCGTCACTTCCACGTCATCGTCATCACTGACGTCGTCTTCAACCACC
GGAAACGCTTCGCCGGAGATCATCAAGTTAAGC

Last Updated on 06.12.2018 16:11