Sequences from confirmation amplicon


Confirmation sequences for line 288B01 and hit At3g10140 are shown.
Line ID 288B01
Locus At3g10140 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3135157
Primers used for PCR HP29 - 8474, show primer details
   
Sequence Name 33-K019175w-022-288-B01C-HP29
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, HP29: 5'-AAATTAACAACAACCTTTGGCAAT-3'
Sequence (A. th genome BLAST matches underlined)
>33-K019175w-022-288-B01C-HP2
CCTCCAACTCCAAGAGCTAGATCAAGGTTGAGGGAACCCGTGGATATCACAGAAACTCTC
CTTTTTCTGTAAAACCGCTGCAATGACAACTTAGAGTCTTTATCAAAATCACCTGAAAGC
TGTGACAATGCTAAATGTAGAGCAGTGTCTTTCTCCGCTACTTTTCTATCATCTGGAACT
TCATCAAGTTCACAATCTGAAGCTTCAACTGACCAAACGAAACACACTCATAAAGACAGG
ATACAGAGTATGGAAATCAAAAGACAAAGGAAATTAGTGATTACCTAAAGACGAAAGATA
ACGATTTTCATAGCCAGAGCAAGCCAAAACGCTTCTTCTTCCATTCAGCTACAAAGCAAG
ATAAGAGTAAACACAAGAGACATTTGTTTAGTCAGGAGCTTGGGCAAATCATCAATGCTA
CTCTAAAGGAGATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 31-K019175w-022-288-B01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>31-K019175w-022-288-B01C-840
ATCTCCTTTAGAGTAGCATTGATGATTTGCCCAAGCTCCTGACTAAACAAATGTCTCTTG
TGTTTACTCTTATCTTGCTTTGTAGCTGAATGGAAGAAGAAGCGTTTTGGCTTGCTCTGG
CTATGAAAATCGTTATCTTTCGTCTTTAGGTAATCACTAATTTCCTTTGTCTTTTGATTT
CCATACTCTGTATCCTGTCTTTATGAGTGTGTTTCGTTTGGTCAGTTGAAGCTTCAGATT
GTGAACTTGATGAAGTTCCAGATGATAGAAAAGTAGCGGAGAAAGACACTGCTCTACATT
TAGCATTGTCACAGCTTTCAGGTGATTTTGATAAAGACTCTAAGTTGTCATTGCAGCGGT
TTTACAGAAAAAGGAGAGTTTCTGTGATATCCACGGGTTCCCTCAACCTTGATCTAGCTC
TTGGAGTTGGAGGATTGCCAAAGGTTG

Last Updated on 23.10.2014 13:25