Sequences from confirmation amplicon


Confirmation sequences for line 290F09 and hit At5g45800 are shown.
Line ID 290F09
Locus At5g45800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18576620
Primers used for PCR DL47 - 8474, show primer details
   
Sequence Name 05-K018490-022-290-F09C-DL47
GenBank Accession LN488153 [GenBank]
Sequencing primer gene specific primer, DL47: 5'-GGTGACGTATTAGCCAATTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018490-022-290-F09C-DL47
CCCATGGATGGGCACTCCAATCTTCCACGTTGGTTTCTCCCGCCGGGAGCTCGTGTAGCC
ATCGGTGAAGATCTCCGTTAGCCATAAATTCATATAATATAAGCTTCTCTTTTCCTAAAA
ATAATAAACCGCAATATTGGTTACGAACAAATAAATATAATACTGATTTTTTGTCACAAG
TATGAATATTTTAAATTATATATGTAAATTAATATTGCATTTCCTAATTCACCCATACCA
AATAAATTCAGTAAATTGTAAATCTTAGTTAAATACTGAAATTAATACAGTGATGCTAGA
CTATTAACTTTCGAATTCACTATTTAAACAGAGTTAATACTGCAAAACATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 06-K018490-022-290-F09C-8409
GenBank Accession LN488599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018490-022-290-F09C-8409
TAGATTTCCCGGACATGTTTTGCAGTATTAACTCTGTTTAAATAGTGAATTCGAAAGTTA
ATAGTCTAGCATCACTGTATTAATTTCAGTATTTAACTAAGATTTACAATTTACTGAATT
TATTTGGTATGGGTGAATTAGGAAATGCAATATTAATTTACATATATAATTTAAAATATT
CATACTTGTGACAAAAAATCAGTATTATATTTATTTGTTCGTAACCAATATTGCGGTTTA
TTATTTTTAGGAAAAGAGAAACTTATATTATATTAATTTATGGGTTACCG

Last Updated on 06.12.2018 16:11