Sequences from confirmation amplicon


Confirmation sequences for line 314B12 and hit At1g79000 are shown.
Line ID 314B12
Locus At1g79000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29721716
Primers used for PCR HF26 - 8474, show primer details
   
Sequence Name 19-K032600-022-314-B12C-8409
GenBank Accession LN494653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K032600-022-314-B12C-8409
TTAAATTGAAATATCCTGTGCACATATCACTCTGACTTGTGACAGGGAGAATCTGAGAAC
CTGCATGACGGTCTTCTGCATTGTTCTGGTATTGATTCTGAAACTGAGAAAGCTGAAATT
GCTCAGGAGTTTGGGAGTGGATAGACTCGCTAGGGTTCTCCATTCCAGGTTCATGTTTAA
CGTGAGTAACCATATCAGAAGCCACATTATTTTGCCCAAAAGCATCGTGACTTATAAATC
TCTGTTGCTGCTGGCTTTGCTGCTTCAATTGCTGTTGATGAAGAAACTGCTGCTGCTGTT
GGTGAA
   
Sequence Name 19-K032600-022-314-B12C-HF26
GenBank Accession LN494654 [GenBank]
Sequencing primer gene specific primer, HF26: 5'-AACCTAATCAATTTCACCAACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K032600-022-314-B12C-HF26
GCATTGAGCAGCAAAGCCAGCAGCAACAGAGATTTATAAGTCACGATGCTTTTGGGCAAA
ATAATGTGGCTTCTGATATGGTTACTCACGTTAAACATGAACCTGGAATGGAGAACCCTA
GCGAGTCTATCCACTCCCAAACTCCTGAGCAATTTCAGCTTTCTCAGTTTCAGAATCAAT
ACCAGAACAATGCAGAAGACCGTCATGCAGGTTCTCAGATTCTCCCTGTCACAAGTCAGA
GTGATATGTGCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FQ12 - 8474, show primer details
   
Sequence Name 92-K035343-022-314-B12C-8409
GenBank Accession LN515163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K035343-022-314-B12C-8409
ATTCCGGAATGNAGCATTTACAATTGAATATATCCTGAACATACTCAAATGTCTAATAGT
ATGTCGAACGAACGGCACATTCAGGAGGATTTCCGACAAAGAATGTCTGGAACGGATGAA
GCTCAGCCCAATAATATGTCTGGAGGCTCAATTATAGGTCAAAATCGTGTCTCGACTACA
TCAGAATCCCTTAATCCCCAAAATCCTACTGCTACCACTTGTAGAAATGGAAATGGCAAC
CGGGATCCGAGATTCAAAAATCAACAGAAATGGCTCCTCTTTCTACGCCATGCTCGCCAC
TGCAAAGCGCCTGAAGGGAAGTGTCCTGATCGAAATTGTGTTACGGTTCAAAAGCTCTGG
AAACACATGGACAGTTGTGCTGCACCTCAATGTTCATATCCTCGTTGCCTCCCCACAAAG
A
   
Sequence Name 92-K035343-022-314-B12C-FQ12
GenBank Accession LN515164 [GenBank]
Sequencing primer gene specific primer, FQ12: 5'-ATTAATCAATGTCTTTGTGGGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K035343-022-314-B12C-FQ12
TTGAGGTGAGCCAACTGTCCATGTGTTTCCAGAGCTTTTGAACCGTAACACAATTTCGAT
CAGGACACTTCCCTTCAGGCGCTTTGCAGTGGCGAGCATGGCGTAGAAAGAGGAGCCATT
TCTGTTGATTTTTGAATCTCGGATCCCGGTTGCCATTTCCATTTCTACAAGTGGTAGCAG
TAGGATTTTGGGGATTAAGGGATTCTGATGTAGTCGAGACACGATTTTGACCTATAATTG
AGCCTCCAGACATATTATTGGGCTGAGCTTCATCCGTTCCAGACATTCTTTGTCGGAAAT
CCTCCTGAATGTGCCGTTCGTTCGACATACTATTAGACATTTGAGTATGTTCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAACTTTATTTCGACGT
GTCTACATTCACGT

Last Updated on 06.12.2018 16:11