Sequences from confirmation amplicon


Confirmation sequences for line 323E06 and hit At1g54210 are shown.
Line ID 323E06
Locus At1g54210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20241588
Primers used for PCR 43ra - 8474, show primer details
   
Sequence Name 87-K115895-0022-323-E06C-43ra
GenBank Accession LN514796 [GenBank]
Sequencing primer gene specific primer, 43ra: 5'-GCGAGAAAGCACTATTCACATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K115895-0022-323-E06C-43ra
GATCATCGCATTCTAAACGATTCATACCAATAGAGGAGAAGCTGTGTTTTTTGTTACCAA
CGAATCAGAATGAAGCTGTCTTCTAAGAAAGTCAATCACTTTGGCAAATTTATCAGTCCC
TGGAATCTATTTACACCAACATTCACAACACATAATGATCAAAAACTTAACAAGCAACGT
TTAGCAAAATGGCTATTCAATTGAATATAGCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 57-K115894-0022-323-E06C-8409
GenBank Accession LN509511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K115894-0022-323-E06C-8409
ATTGAATATATCCTGGCTATATTCAATTGAATAGCCATTTTGCTAAACGTTGCTTGTTAA
GTTTTTGATCATTATGTGTTGTGAATGTTGGTGTAAATAGATTCCAGGGACTGATAAATT
TGCCAAAGTGATTGACTTTCTTAGAAGACAGCTTCATTCTGATTCGTTGGTAACAAAAAA
CACAGCTTCTCCTCTATTGGTATGAATCGTTTAGAATGCGATGATCTTGGTCTGACATTG
CTCTTTTGTTTGTAAGTTTGTCTATGTGAATAGTGCTTTCTCG

Last Updated on 06.12.2018 16:11