Sequences from confirmation amplicon


Confirmation sequences for line 325E04 and hit At3g12690 are shown.
Line ID 325E04
Locus At3g12690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4030948
Primers used for PCR DL33 - 8474, show primer details
   
Sequence Name 31-K018475-022-325-E04C-DL33
GenBank Accession LN499954 [GenBank]
Sequencing primer gene specific primer, DL33: 5'-TCTGATGAGTTTGTTGACAGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K018475-022-325-E04C-DL33
CGTCTGAAGTGCTATCTGGCTTTGGAGTATCTACACATGCTGGGGGTTGTGTACAGAGAC
TTAAAGCCAGAGAACATCCTTGTTCGAGATGAAGGCCACATAATGCTCTCAGACTTCGAC
CTCTCCCTCCGTTGCACCTTTAATCCGACCCTCGTTAAATCCTCCTCAGTCTGCAGCGGG
GGAGGTGCTATCCTCAATGAGGAGTTTGCTGTCAATGGCTGCATGCATCCATCCGCCTTT
CTTCCCCGCCTACTCCCTTCCAAGAAAACCCGTAAAGCGAAATCAGACTCTGGTCTCGGT
GGCCTCTCTATGCCAGAGCTCATGGCAGAGCCAACAGATGTGCGGTCAATGTCCTTTGTG
GGCACACACGAGTACCTAGCTCCAGAGATCATACGTGGCGAGGGACATGGCAGCGCTGTG
GACTGGTGGACATTCGGGATCTTCCTTTATGAGCTCCTCCATGGGACCACCCCGTTCAAG
GGGCAGGGAAACAGAGCCACGTTGCACAATGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATG
   
Sequence Name 32-K018475-022-325-E04C-8409
GenBank Accession LN500381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K018475-022-325-E04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACATTGTGCAACGTGGCTCTGTTTCCCTGC
CCCTTGAACGGGGTGGTCCCATGGAGGAGCTCATAAAGGAAGATCCCGAATGTCCACCAG
TCCACAGCGCTGCCATGTCCCTCGCCACGTATGATCTCTGGAGCTAGGTACTCGTGTGTG
CCCACAAAGGACATTGACCGCACATCTGTTGGCTCTGCCATGAGCTCTGGCATAGAGAGG
CCACCGAGACCAGAGTCTGATTTCGCTTTACGGGTTTTCTTGGAAGGGAGTAGGCGGGGA
AGAAAGGCGGATGGATGCATGCAGCCATTGACAGCAAACTCCTCATTGAGGATAGCACCT
CCCCCGCTGCAGACTGAGGAGGATTTAACGAGGGTCGGATTAAAGGTGCAACGGAGGGAG
AGGTCGAAGTCTGAGAGCATTATGTGGCCTTCATCTCGAACAAGGATGTTCTCTGGCTTT
AAGTCTCTGTACACAACCCCCAGCATGTGTAGATACTCCAAAGCCAATAGCACTTCAGAC
GCGTAGAACCTGTCAAC

Last Updated on 06.12.2018 16:11