Sequences from confirmation amplicon


Confirmation sequences for line 328D05 and hit At1g30100 are shown.
Line ID 328D05
Locus At1g30100 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10571860
Primers used for PCR AM02 - 8474, show primer details
   
Sequence Name 43-K021644-022-328-D05C-8409
GenBank Accession LN504693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>43-K021644-022-328-D05C-8409
TCGGAATGAGCATTTACAATTGAATATATCCTGAAGCTATTCGACGGTGACGGTATGGTT
CACGCCGTTAAAATCACTAACGGAGACGCGAGTTACTCGTGCCGGTTTACGGAAACCGAG
AGATTGGTTCAAGAGAAACAACTCGGTTCTCCGATTTTCCCTAAAGCTATAGGTGAGCTA
CATGGTCACTCTGGAATCGCACGGTTGATGCTATTTTACGCACGTGGTTTATTCGGTTTA
TTAAATCACAAAAACGGAACCGGAGTTGCTAACGCCGGTTTGGTTTACTTCCACGACCGG
TTATTAGCTATGTCTGAAGATGATCTACCTTACCAAGTTCGTGTCACTGACAATGGCGAT
TTAGAGACCATCGGAAGATTCGATTTCGACGGACAACTAAGCTCCGCCATGATCGCTCAC
CCGAAGATTGATCCGGTAACGAAGGAGCTATTTGCGTTGAGCTACGACGTCGTTAAGAAA
CCGTATTTGAAATACTTTAAATTCTCGCCGGAAGGTGAGAAATCACCGGACGTTGAGATT
CCTCTCGCCAGTCCGACGATGATGCACGATTTCGCGATCACTGAGAATTTCGTTGTGATT
CCGGATCAACAAGTTGTGTTTAAGCTCTCCGATATGTTTCTTGGGAAATCTCCGGTTAAA
TACGACGGAGAGAAAATTTCCCGGTTTGGAATTTT
   
Sequence Name 44-K021644-022-328-D05C-AM02
GenBank Accession LN505041 [GenBank]
Sequencing primer gene specific primer, AM02: 5'-CTTTAGCGTTTCTAGGCAAAATTC-3'
Sequence (A. th genome BLAST matches underlined)
>44-K021644-022-328-D05C-AM02
CGTCGTATTTACCGGAGATTTCCCAGAAACATATCGGAGAGCTTAACCAACTTGTTGATC
CGGAATCACAACGAAATTCTCAGTGATCGCGAAATCGTGCATCATCGTCGGACTGGCGAG
AGGAATCTCAACGTCCGGTGATTTCTCACCTTCCGGCGAGAATTTAAAGTATTTCAAATA
CGGTTTCTTAACGACGTCGTAGCTCAACGCAAATAGCTCCTTCGTTACCGGATCAATCTT
CGGGTGAGCGATCATGGCGGAGCTTAGTTGTCCGTCGAAATCGAATCTTCCGATGGTCTC
TAAATCGCCATTGTCAGTGACACGAACTTGGTAAGGTAGATCATCTTCAGACATAGCTAA
TAACCGGTCGTGGAAGTAAACCAAACCGGCGTTAGCAACTCCGGTTCCGTTTTTGTGATT
TAATAAACCGAATAAACCACGTGCGTAAAATAGCATCAACCGTGCGATTCCAGAGTGACC
ATGTAGCTCACCTATAGCTTTAGGGAAAATCGGAGAACCGAGTTGTTTCTCTTGAACCAA
TCTCTCGGTTTCCGTAAACCGGCACGAGTAACTCGCGTCTCCGTTAGTGATTTTAACGGC
GTGAACCATACCGTCACCGTCGAATAGCTTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATT

Last Updated on 23.10.2014 13:25