Sequences from confirmation amplicon


Confirmation sequences for line 335D04 and hit MWD22 are shown.
Line ID 335D04
Locus MWD22 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20834233
Primers used for PCR JZ18 - 8474, show primer details
   
Sequence Name 09-K037813-022-335-D04C-8409
GenBank Accession LN490091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K037813-022-335-D04C-8409
CCATTTCAATTGAAGCCATGTAGATTTCTAAAATGTCTTCTTCTTTTTTCATTTTATGTT
TGTATGTGATGAATTATAAAGGAAAAAAGAATTACTGATCAGAAAATTTCGTACGATCTG
TTTAATGAAGTCAATTTCTGATTTAAGAGACTATAAAAGTAACAAAATTTCATAAACTAT
TATAAAATCTGAGTAAGATCCTTAATCCAAAGTTTAATACTAGTGATAATGGGAACTTAT
GATAGCGGATAGTTGCACGCACGCTAAGCTACGCCTACGCGTGGAGTGTTAATGGAATTG
ATTGTGACATAAGTTTCAAGTTATTTAACCAATAACGTGTTTATAAGGGCTGATTAATTA
TACCATTAATGTATTATGACCAAAACTATCTCACGGTTTGGAGAAGAGAAAAAGAAAGAG
TTATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCCCAGCGTTTTTATAAAATGA
AAGTACATTTTGATAAAACGACCAATTACGATCCGTCGTAATTTTTGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATGTCGACGTGTCTACATTCACGTCCAA
   
Sequence Name 01-K037813-022-335-D04C-JZ18
GenBank Accession LN486487 [GenBank]
Sequencing primer gene specific primer, JZ18: 5'-CTCCAAACGTGAGATAGTTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K037813-022-335-D04C-JZ18
ATTATCAGCCCTTATAAACACGTTATTGGTTAAATAACTTGAAACTTATGTCACAATCAA
TTCCATTAACACTCCACGCGTAGGCGTAGCTTAGCGTGCGTGCAACTATCCGCTATCATA
AGTTCCCATTATCACTAGTATTAAACTTTGGATTAAGGATCTTACTCAGATTTTATAATA
GTTTATGAAATTTTGTTACTTTTATAGTCTCTTAAATCAGAAATTGACTTCATTAAACAG
ATCGTACGAAATTTTCTGATCAGTAATTCTTTTTTCCTTTATAATTCATCACATACAAAC
ATAAAATGAAAAAAGAAGAAGACATTTTAGAAATCTACATGGCTTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11