Sequences from confirmation amplicon


Confirmation sequences for line 337A11 and hit At2g18420 are shown.
Line ID 337A11
Locus At2g18420 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7994381
Primers used for PCR MR92 - 8474, show primer details
   
Sequence Name 39-K017464-022-337-A11C-MR92
GenBank Accession LN503184 [GenBank]
Sequencing primer gene specific primer, MR92: 5'-ATGATACATGGGGTTTGATTGTTT-3'
Sequence (A. th genome BLAST matches underlined)
>39-K017464-022-337-A11C-MR92
TTGCTTTTTTGTCCGGTTTGATCTTGAATATGTTACATTACACTGCAGTATACTGTTGAA
GATATGATTGATGCTTCTTTTGTTGAAGCTCATGTTATATAGAATATAGAATAATAGTTG
AACGATTAATTATGCGGTGAATTTATTTGCCTCGAAGAGTCAAGACTCAAGACTATTATT
AACTTTATAGAGGTTATACCTTTCTAGGAATCTTGTACAAAAATGTAATAAAAATCGTAC
TGTTACTTCTATATATAATACATTCAACCCGGAAACATTAATTAAACGTAATTTCATATA
CTATCTTTCATAATTTTATTGGTATTGTTGGTAACGTTACATATTCAATCGCTCTCAGTT
TTACGTCATAGTTCATTAAGTCCCCTCCAATTCTGTTGTTACCATATTTAAACCCATTGC
CTAACTTCCATGAGTAAAAAAAGAAAACTCTTTTACTTCTCTCTTCATTCTTTATTTTAT
ATGCAAGTACGATGCCTATTTAGTGAATTGTATAATGAAAAACAATACCTTTTCAGACCT
AATGTTAACCAGGAACCAAACCAACAAGTACAAATTGGATAAACACTAAAACAAAGATCG
AAGAGTTGCACATGACGTATAACATTCTCTTTCTTTTGCTTTTTAATAATT
   
Sequence Name 40-K017464-022-337-A11C-8409
GenBank Accession LN503545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>40-K017464-022-337-A11C-8409
AATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGTTATTGT
GGTTATGAGAGTAATTGTGGTTATTTTCTTGGGAATTATTAAAAAGCAAAAGAAAGAGAA
TGTTATACGTCATGTGCAACTCTTCGATCTTTGTTTTAGTGTTTATCCAATTTGTACTTG
TTGGTTTGGTTCCTGGTTAACATTAGGTCTGAAAAGGTATTGTTTTTCATTATACAATTC
ACTAAATAGGCATCGTACTTGCATATAAAATAAAGAATGAAGAGAGAAGTAAAAGAGTTT
TCTTTTTTTACTCATGGAAGTTAGGCAATGGGTTTAAATATGGTAACAACAGAATTGGAG
GGGACTTAATGAACTATGACGTAAAACTGAGAGCGATTGAATATGTAACGTTACCAACAA
TACCAATAAAATTATGAAAGATAGTATATGAAATTACGTTTAATTAATGTTTCCGGGTTG
AATGTATTATATATAGAAGTAACAGTACGATTTTTATTACATTTTTGTACAAGATTCCTA
GAAAGGTATAACCTCTATAAAGTTAATAATAGTCTTGAGTCTTGACTCTTCGAGGCAAAT
AAATTCACCGCATAATTAATCGTTCAACTATTATTCTATATTCTATATAACATGAGCTTC
AAACAAAGAAGCATCAATCATATCTTCAACAGTATACTGCAGTGTAATGTAACAT

Last Updated on 23.10.2014 13:25