Sequences from confirmation amplicon


Confirmation sequences for line 341D04 and hit At4g25100 are shown.
Line ID 341D04
Locus At4g25100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12885129
Primers used for PCR JH05 - 8474, show primer details
   
Sequence Name 02-K025266-022-341-D04C-JH05
GenBank Accession LN486878 [GenBank]
Sequencing primer gene specific primer, JH05: 5'-CAGCACTTACAACAATGGTGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K025266-022-341-D04C-JH05
CTTTCAACAACGCTGCTCAGGTGATAATTAACAAACCTATTTTTAGTTTGAGATTATAAA
ATGGGTGTGTGATTGGTTAGCTGATGATCCATTGGTTATGTTTATAGGCGTGGAACCACG
AGTTCTTCTGGGAGTCAATGAAACCAGGTGGTGGAGGAAAACCATCAGGAGAGCTTCTTG
CTTTGCTTGAAAGAGATTTCACTTCTTATGAGAAGTTCTATGAAGAGTTCAATGCTGCTG
CAGCCACTCAGTTTGGAGCTGGCTGGGCCTGGCTTGCTTGTAAGTGTTTCAAATGTTTAA
GAGCTTTAGATAAATATATTAACTTCTTCCTTACTCTTATCTCAATGCTTTCAGATTCAA
ATGAAAAACTCAAAGTAGTGAGTATGATGGATCAGCAATTGTAAATTGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 01-K025266-022-341-D04C-8409
GenBank Accession LN486422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K025266-022-341-D04C-8409
GTAGATTTCCCGGACATGAAGCAATTTACAATTGCTGATCCATCATACTCACTACTTTGA
GTTTTTCATTTGAATCTGAAAGCATTGAGATAAGAGTAAGGAAGAAGTTAATATATTTAT
CTAAAGCTCTTAAACATTTGAAACACTTACAAGCAAGCCAGGCCCAGCCAGCTCCAAACT
GAGTGGCTGCAGCAGCATTGAACTCTTCATAGAACTTCTCATAAGAAGTGAAATCTCTTT
CAAGCAAAGCAAGAAGCTCTCCTGATGGTTTTCCTCCACCACCTGGTTTCATTGACTCCC
AGAAGAACTCGTGGTTCCACGCCTATAAACATAACCAATGGATCATCAGCTAACCAATCA
CACACCCATTTTATAATCTCAAACTAAAAATAGGTTTGTTAATTATCACCTGAGCAGCGT
TGTTGAAAGCAGGGAGGAGATCACCTTTGT

Last Updated on 06.12.2018 16:11