Sequences from confirmation amplicon


Confirmation sequences for line 349D04 and hit At1g01520 are shown.
Line ID 349D04
Locus At1g01520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:192109
Primers used for PCR AY19 - 8474, show primer details
   
Sequence Name 17-K018334-022-349-D04C-AY19
GenBank Accession LN493663 [GenBank]
Sequencing primer gene specific primer, AY19: 5'-CCATTCCGTATAAAAACCTAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K018334-022-349-D04C-AY19
AATATTACAAAAAAGCTAGTGACTTTCTACACAGGCTTTATCTGTCTTCTAAGACGTTCT
AAACTATTGAGACTTATATGTACAACCGGTTCATTCATTCATATCCCATGAGACTACCAT
TAGAGACTTCTCTCTATCGTTTAGTTCTAACTGGCGTTGTAAGATGATATCAACTTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGT
   
Sequence Name 18-K018334-022-349-D04C-8409
GenBank Accession LN494108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K018334-022-349-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAGTTGATATCATCTTACAACGCCAGT
TAGAACTAAACGATAGAGAGAAGTCTCTAATGGTAGTCTCATGGGATATGAATGAATGAA
CCGGTTGTACATATAAGTCTCAATAGTTTAGAACGTCTTAGAAGACAGATAAAGCCTGTG
TAGAAAGTCACTAGCTTTTTTGTAATATTCAGTTGTTGCTTAGGTTTTTATACGGAATGG
ACTAATGACATAACTAGTTTTGAAAATTACTTCAGATAAAGCCTGTGTAGAAAGTCACTA
GCTTTTTTGTAATATTCAGTTGTTGCTTAGGTTTTTATACGGAATGG

Last Updated on 06.12.2018 16:11