Genotyping and primer details


Genotyping and primer details for 349D04 - At1g01520
Line ID 349D04
Locus At1g01520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:192109 of line 349D04 Chr1:192109
   
Insertion allele amplicon 8474 - AY19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY19: 5'-CCATTCCGTATAAAAACCTAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:192316
   
wt amplicon (over InsPos) FN28 - AY19
wt amplicon start Chr1:191646
wt amplicon end Chr1:192316
wt amplicon size 777 bp
Locus specific primer AY19: 5'-CCATTCCGTATAAAAACCTAAGCA-3'
2nd locus specific primer FN28: 5'-CGTTACAGAGACAAATGACCAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11