Sequences from confirmation amplicon


Confirmation sequences for line 349G11 and hit At1g63650 are shown.
Line ID 349G11
Locus At1g63650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23602505
Primers used for PCR MH01 - 8474, show primer details
   
Sequence Name 58-K018157-022-349-G11C-8409
GenBank Accession LN509572 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K018157-022-349-G11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATCACTAATCACATCC
ATTATCTACATGGCTAATCACATCCATTGTTGAGATCACTAATCACATCCATTATCTCAA
GCAATATCCCTTCTCTCCAAGCGCATCTAAGCTCAATAACCACCTCGTTACCTAATGAAC
TGATCCTTAAGTTATCCGTTAGACCAGCATAACCGATATCAGCTGGTTCATCTTCTCCTA
CATTCACATCACTCCCCTTCCTTTTGCTGTTCATACAATTCGCTGATGCTCTTTCCTCCT
CATCATCCGGTTTCTTCCTCTTCATCATCGTTATCCGTGTCTCTGTATCAGCAGATTCTC
TACAAGATTCCAACTCTTGAACCCGTTTCTGTAAATCTTGAAGATACTCAATTGTATCAT
CAAGAATCGACACTTTATCAATCTGTAGAATCAGCAAGAGACTCAAGCTTAGTTCCTTCA
GAGCATTATATTTAAACATGTCTTCCTATTATCAAGAAATAAACATAAAACGTTACTGAA
ACAGATTTGCTAGAATCGGTTACCTTACTAATTGAGGGAATGATTGATCTCAATGTCATA
AACCGTTCATTCAATTTCTCGCGGCGTTTCTTCTCGGACAAGGCATGGTTCCCGGTTTCC
TCTGGTGTGTCCGGTAACAACT
   
Sequence Name 59-K018158-022-349-G11C-MH01
GenBank Accession LN509855 [GenBank]
Sequencing primer gene specific primer, MH01: 5'-TAACCATGTAAATATGGACGACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K018158-022-349-G11C-mh01
TTCATTACCAAGGCGTGATATCGACGATTTTCAAAACAACGCATCAGCTAATACTCGGAC
CGCAGTTTCAGAACTTCGATAAGCGGTCTAGCTTCACAAGGTGGAAGCGATCATCATCTG
TGAAAACATTGGGAGAGAAATCGCAGAAGATGATAAAGAAGATACTCTTCGAGGTTCCTT
TGATGAACAAGAAAGAAGAGTTGTTACCGGACACACCAGAGGAAACCGGGAACCATGCCT
TGTCCGAGAAGAAACGCCGCGAGAAATTGAATGAACGGTTTATGACATTGAGATCAATCA
TTCCCTCAATTAGTAAGGTAACCGATTCTAGCAAATCTGTTTCAGTAACGTTTTATGTTT
ATTTCTTGATAATAGGAAGACATGTTTAAATATAATGCTCTGAAGGAACTAAGCTTGAGT
CTCTTGCTGATTCTACAGATTGATAAAGTGTCGATTCTTGATGATACAATTGAGTATCTT
CAAGATTTACAGAAACGGGTTCAAGAGTTGGAATCTTGTAGAGAATCTGCTGATACAGAG
ACACGGATAACGATGATGAAGAGGAAGAAACCGGATGATGAGGAGGAAAGAGCATCAGCG
AATTGTATGAACAGCACAAGGAAGGGGAGTGATGTGAATGTAGGAGAAGATGAACCAGCT
GATATCGGTTATGCTGGTCTAACGGATAA

Last Updated on 06.12.2018 16:11