Genotyping and primer details


Genotyping and primer details for 349G11 - At1g63650
Line ID 349G11
Locus At1g63650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23602505 of line 349G11 Chr1:23602505
   
Insertion allele amplicon 8474 - MH01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MH01: 5'-TAACCATGTAAATATGGACGACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23601688
   
wt amplicon (over InsPos) 07rd - MH01
wt amplicon start Chr1:23601688
wt amplicon end Chr1:23603101
wt amplicon size 1414 bp
Locus specific primer MH01: 5'-TAACCATGTAAATATGGACGACGA-3'
2nd locus specific primer 07rd: 5'-AATGTTTTTGGTCGAACTTGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11