Sequences from confirmation amplicon


Confirmation sequences for line 350G05 and hit At2g45310 are shown.
Line ID 350G05
Locus At2g45310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18683784
Primers used for PCR MP21 - 8474, show primer details
   
Sequence Name 51-K020436-022-350-G05C-8409
GenBank Accession LN507364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K020436-022-350-G05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGCCTTTTGCTGG
CCTTCACTTTCAATTGTCTCTCCAATATCCTCACCAGATCTGAAACCGGCACCGGCGACG
TGTTTCCGAGGTTAAACACTCTCAATTGCGCCGGACCTCTCTTCTTCCCTCCACTCCCTG
TACTCTTCTCCGCCGTATCTAGCGCCGCTAAACATCCCTTCACTATATCGTCTATGTAAG
TGAAATCCCTAGCCACCGTTCCGTGATTCGCCGATTCGAATATCGAAATCGATTTGCCTT
TTAGAATATCTTTAGTGAAGAAGAAATACGCCATGTCTGGTCTTCCCCAAGGTCCATACA
CGGTGAAAAATCTT
   
Sequence Name 52-K020436-022-350-G05C-MP21
GenBank Accession LN507681 [GenBank]
Sequencing primer gene specific primer, MP21: 5'-TTAAGATTTTTCACCGTGTATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K020436-022-350-G05C-MP21
GGGGAAGACCAGACATGGCGTATTTCTTCTTCACTAAAGATATTCTAAAAGGCAAATCGA
TTTCGATATTCGAATCGGCGAATCACGGAACGGTGGCTAGGGATTTCACTTACATAGACG
ATATAGTGAAGGGATGTTTAGCGGCGCTAGATACGGCGGAGAAGAGTACAGGGAGTGGAG
GGAAGAAGAGAGGTCCGGCGCAATTGAGAGTGTTTAACCTCGGAAACACGTCGCCGGTGC
CGGTTTCAGATCTGGTGAGGATATTGGAGAGACAATTGAAAGTGAAGGCCAGCAAAAGGC
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11