Sequences from confirmation amplicon


Confirmation sequences for line 355B05 and hit At5g26680 are shown.
Line ID 355B05
Locus At5g26680 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9314095
Primers used for PCR HP25 - 8474, show primer details
   
Sequence Name 19-K019175w-022-355-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>19-K019175w-022-355-B05C-840
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACTGAGAATTAAGACC
TTTCCTGACTTGCAAAGTGCAGCACATTGCGCTTCAGCCTCGGAAGTGGCCTGTATGTAA
GAAGCAACATCAGTCTTCTTTTCAAACTTTTAGCCATTTCATATCATAAACAAAAAGACG
AGTTAATAGAAGAGATTTAGACCCTCGGGACTCTGCACATAACATAAGTACCTCAACAAC
AGGCACCCCCATGAGTCTCAAGAGTCTTTTGCAGTCATCATTATGCTGTTTTGTCACCTG
GAGAGTGGAAATTTATACATATATTAATACGAGTCCTAGATTCAATTGCTTTGAAGTGGA
TGCCATAAAAACTAAGGAGTCCAACGATTCATAGATGAAAAAATAATATACTACCTTCAC
AGTTCGTTTGCTGTACTTCTCAATGTCTTCCTTGTTTCCTGCCTATCAGCACAGAAAAGC
TTCTAAATTTATGCTCTTCCGGTAATAAAATAAAGCTAGGCAAGGCAAGTAAGAGTGACA
AACCTCAATTGCACCAGTCAAATCTGCAGTTGCATCAGCTCTCTTGGAGTAACTGAATCA
AGAGTAGAAAGCAACAGTTCA
   
Sequence Name 20-K019175w-022-355-B05C-HP25
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, HP25: 5'-TGGCTAAAGTGAGTTTCTGAACTG-3'
Sequence (A. th genome BLAST matches underlined)
>20-K019175w-022-355-B05C-HP2
CTTTCTACTCTTGATTCAGTTACTCCAAGAGAGCTGATGCAACTGCAGATTTGACTGGTG
CAATTGAGGTTTGTCACTCTTACTTGCCTTGCCTAGCTTTATTTTATTACCGGAAGAGCA
TAAATTTAGAAGCTTTTCTGTGCTGATAGGCAGGAAACAAGGAAGACATTGAGAAGTACA
GCAAACGAACTGTGAAGGTAGTATATTATTTTTTCATCTATGAATCGTTGGACTCCTTAG
TTTTTATGGCATCCACTTCAAAGCAATTGAATCTAGGACTCGTATTAATATATGTATAAA
TTTCCACTCTCCAGGTGACAAAACAGCATAATGATGACTGCAAAAGACTCTTGAGACTCA
TGGGGGTGCCTGTTGTTGAGGTACTTATGTTATGTGCAGAGTCCCGAGGGTCTAAATCTC
TTCTATTAACTCGTCTTTTTGTTTATGATATGAAATGGCTAAAAGTTTGAAAAGAAGACT
GATGTTGCTTCTTACATACAGGCCACTTCCGAGGCTGAAGCGCAATGTGCTGCACTTTGC
AAGTCAGGAAAGGTCTTAATTCTCAGTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTT

Last Updated on 23.10.2014 13:25