Sequences from confirmation amplicon


Confirmation sequences for line 377G03 and hit At1g58380 are shown.
Line ID 377G03
Locus At1g58380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21689265
Primers used for PCR 46oh - 8474, show primer details
   
Sequence Name 80-K114716-0022-377-G03C-46oh
GenBank Accession LN513981 [GenBank]
Sequencing primer gene specific primer, 46oh: 5'-GATAGCTTTAGCAGTACAATGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K114716-0022-377-G03C-46oh
TTGGTTTGCGAAATTAAGCCTTAAAAAAGCCCACAGATATTTGTCAAAGAATCTTAATAA
CCTTTTTGTTGCTCAAGCGCCGTTCCTAGGGTTTTTCTCTGAGGAAGAAGCGTTTCATTT
CTCTGAATTTCATCGAAAATGGCGGAAAGAGGAGGAGAAGGCGGCGCAGAGCGTGGCGGT
GACCGTGGTGACTTCGGACGTGGATTCGGCGGTGGACGTGGAGGTGGCCGTGGCCGTGAT
CGTGGTCCAAGAGGCCGTGGAAGACGTGGAGGCCGTGCCTCGGAAGAAAAAGAAAAAGAA
ACAAACCAATTTACCAATAAAATACATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACAGTTATA
   
Sequence Name 32-K114715-0022-377-G03C-8409
GenBank Accession LN500692 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114715-0022-377-G03C-8409
TTATTGGTAATTGGTTTGTTTCTTTTTCTTTTTCTTCCGAGGCACGGCCTCCACGTCTTC
CACGGCCTCTTGGACCACGATCACGGCCACGGCCACCTCCACGTCCACCGCCGAATCCAC
GTCCGAAGTCACCACGGTCACCGCCACGCTCTGCGCCGCCTTCTCCTCCTCTTTCCGCCA
TTTTCGATGAAATTCAGAGAAATGAAACGCTTCTTCCTCAGAGAAAAACCCTAGGAACGG
CGCTTGAGCAACAAAAAGGTTATTAAGATTCTTTGACAAATATCTGTGGGCTTTTTTAAG
GCTTTAATTTCTGCAAACCAAATATTGGGCCTTTTGCTATTCTTCTTACCATTGCCCATT
GTACTGCTAAAGCTAT

Last Updated on 06.12.2018 16:11