Sequences from confirmation amplicon


Confirmation sequences for line 401F09 and hit At1g05570 are shown.
Line ID 401F09
Locus At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1655315
Primers used for PCR VL24 - 8474, show primer details
   
Sequence Name 43-K020889-022-401-F09C-8409
GenBank Accession LN504690 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K020889-022-401-F09C-8409
GTAGATTTCCCGGACATGAAGCCATTATATCAATTGAAACTCCGGTTTTTCTCAGACCAT
AAAGAATTGGTTTGGGGGACACAGCCATAATTCACCTTCTCTTTTCATAGTGGCAATTCT
TATCTACTTATCCCCGAACATGCTTTCTGCGTTGCTGTTTTTGTTTCCATTCATTCGGCG
CTATCTTGAAAGGTCTGACTATAAGATAATGATGCTGATGATGTGGTGGTCTCAGGTAGT
TTATTCATGTGCTTATATCTTGAAAAATGCTAGTCCATGAATGTTAAAGTGAAAAGATGG
TCTTATCATGGTTCTTTGTGATACGGCCTCTGTCTGTATATAAGAAGGGGTATGCATGAA
AACGCTTTGTCACTTTTCATGTAAGGACCCTAAAACTTATAGGTACGAAAGTCAGTTGTT
CTCTTCATCAC
   
Sequence Name 44-K020889-022-401-F09C-VL24
GenBank Accession LN505040 [GenBank]
Sequencing primer gene specific primer, VL24: 5'-CTTGCATATTGCTCATGCTAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K020889-022-401-F09C-VL24
GTGTAATTAGGAATACAAGTAAATGTGATACCTCAGCGTAATAACTGAATGCCAGCTTTG
AAATCAGCAGAACGATCCAAAACATTGTGTACCTAGAAGTCATTTTGTGCATCATCGTTA
AGCAGTGCTGAAAAAAACAACCTACATTCGAACCTATAAGTTTTAGGGTCCTTACTTGAA
AAGTGACAAAGCGCTCTCATGCATACCCCTTCCTATATACAGACGAGGCTGTATCACAAA
GAACCATGATAAGACCATCTTTTCACTTTAACATTCATGGACTAGCATTTTTCAAGATAT
AAGCACATGAATAAACTACCTGAGACCACCACATCATCAGCATCATTATCTTATAGTCAG
ACCTTTCAAGATAGCGCCGAATGAATGGAAACAAAAACAGCAACGCAGAAAGCATGTTCG
GGGATAAGTAGATAAGAATTGCCACTATGAAAAGAGAAGGTGAATTATGGCTGTGTCCCC
CAAACCAATTCTTTATGGTCTGAGAAAAACCGGAGTTTCAATTGATATAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11